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PXD023901-1

PXD023901 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA proteomics data analysis protocol using Open-pFind
DescriptionHigh-throughput proteomics based on mass spectrometry (MS) analysis has permeated biomedical science and propelled numerous research projects. pFind 3 is a database search engine for high-speed and in-depth proteomics data analysis (Chi et al. 2018). pFind 3 features a swift open search workflow that is adept at uncovering less obvious information such as unexpected modifications or mutations that would have gone unnoticed using a conventional data analysis pipeline. In this protocol, we provide step-by-step instructions to help users mastering various types of data analysis using pFind 3 in conjunction with pParse for data preprocessing and if needed, pQuant for quantitation. This streamlined pParse-pFind-pQuant workflow offers exceptional sensitivity, precision, and speed. It can be easily implemented in any laboratory in need of identifying peptides, proteins, or post-translational modifications, or of quantitation based on 15N-labeling, SILAC-labeling, or TMT/iTRAQ labeling.
HostingRepositoryiProX
AnnounceDate2021-01-31
AnnouncementXMLSubmission_2021-01-31_21:18:21.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuangcan Shao
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-31 21:17:38ID requested
12021-01-31 21:18:21announced
22023-06-11 20:13:55announced2023-06-12: Update publication information.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Open-pFind, protein identification, protein quantitation
Contact List
Meng-Qiu Dong
contact affiliationNational Institute of Biological Sciences
contact emaildongmengqiu@nibs.ac.cn
lab head
Guangcan Shao
contact affiliationNational Institute of Biological Sciences
contact emailshaoguangcan@nibs.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI