PXD023829

PXD023829 is an original dataset announced via ProteomeXchange.

Title	Multilayered Omics Revealsl Sex- and Depot-Dependent Adipose Progenitor Cell Heterogeneity
Description	White adipose tissue (WAT) harbors functionally diverse subpopulations of adipose progenitor cells that differentially impact tissue plasticity in a sex- and depot-dependent manner. To date, the molecular basis of this cellular heterogeneity has not been fully defined. Here, we describe a multilayered omics approach to dissect adipose progenitor cell heterogeneity from in three dimensions: progenitor subpopulation, sex, and anatomical localization. We applied state-of-the-art mass spectrometry methods to quantify 4870 proteins in eight different stromal cell populations from perigonadal and inguinal WAT of male and female mice and acquired transcript expression levels of 15477 genes using RNA-seq. Notably, our data highlight the molecular signatures defining sex differences in PDGFR+ preadipocyte differentiation and identify regulatory pathways that functionally distinguish adipose tissue PDGFRb+ subpopulations. The data are freely accessible as a resource at "Pread Profiler. Together, the multilayered omics analysis provides unprecedented insights into adipose stromal cell heterogeneity.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:37:22.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Midy Wu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-26 02:19:24	ID requested
1	2023-03-10 21:23:10	announced
⏵ 2	2023-11-14 08:37:23	announced	2023-11-14: Updated project metadata.

Shan B, Barker CS, Shao M, Zhang Q, Gupta RK, Wu Y, Multilayered omics reveal sex- and depot-dependent adipose progenitor cell heterogeneity. Cell Metab, 34(5):783-799.e7(2022) [pubmed]

submitter keyword: Multilayered omics, adipogenesis, inflammation, preadipocytes, PPAR., adipocyte precursor cells, cellular heterogeneity

Yibo Wu
contact affiliation	RIKEN Center for Integrative Medical Sciences
contact email	yibo.wu@riken.jp
lab head
Midy Wu
contact affiliation	RIKEN
contact email	wyb0828@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD023829

PRIDE project URI

• PRIDE
  1. PXD023829
     1. Label: PRIDE project
     2. Name: Multilayered Omics Revealsl Sex- and Depot-Dependent Adipose Progenitor Cell Heterogeneity