PXD023760-1

PXD023760 is an original dataset announced via ProteomeXchange.

Title	Tropism of SARS-CoV-2 in commonly used laboratory cell-lines and their proteomic landscape during infection
Description	The present pandemic by Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is driving increased research activities in order to understand the basic biology of the virus and determining effective therapeutic strategies. The commonly used laboratory cell-lines of human origin are the first line of experimental models to study the pathogenicity and performing antiviral assays. Thus, to find a suitable cell model to study SARS-CoV-2, we assessed the tropism and cytopathogenicity of the first Swedish isolate of SARS-CoV-2 in six different cell-lines of human origin and compared their growth characteristics to other globally isolated strains. Overall, majority of the strains showed high to moderate level of susceptibility in Caco2, Huh7, Calu 3 and 293FT cell-lines and in Caco2 cells the virus can achieve high titers in absence of any prominent cytopathic effect. The protein expression profile during SARS-CoV-2 infection revealed cell-type specific regulation of cellular pathways. Type-I interferon signaling was identified as the common dysregulated cellular response in Caco2, Calu-3 and Huh7 cells. Overall, cell-type specific variability was noted with respect to cytopathogenicity, susceptibility and cellular response to SARS-CoV-2. This study provides important clues regarding SARS-CoV-2 pathogenesis and can represent as a guide for future studies.
HostingRepository	PRIDE
AnnounceDate	2021-04-14
AnnouncementXML	Submission_2021-04-14_00:36:55.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JIMMY RODRIGUEZ
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-22 08:39:28	ID requested
⏵ 1	2021-04-14 00:36:55	announced

Dataset with its publication pending

ProteomeXchange project tag: Covid-19

submitter keyword: SARS-CoV-2, cellular tropism, cytopathogenicity, cell-lines, ACE-2, TMPRSS2, Type-I Intereferon, proteomics

Akos Vegvari
contact affiliation	Division of Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
contact email	akos.vegvari@ki.se
lab head
JIMMY RODRIGUEZ
contact affiliation	Division of Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact email	esneider007@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD023760

PRIDE project URI

• PRIDE
  1. PXD023760
     1. Label: PRIDE project
     2. Name: Tropism of SARS-CoV-2 in commonly used laboratory cell-lines and their proteomic landscape during infection