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PXD023760

PXD023760 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTropism of SARS-CoV-2 in commonly used laboratory cell-lines and their proteomic landscape during infection
DescriptionThe present pandemic by Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is driving increased research activities in order to understand the basic biology of the virus and determining effective therapeutic strategies. The commonly used laboratory cell-lines of human origin are the first line of experimental models to study the pathogenicity and performing antiviral assays. Thus, to find a suitable cell model to study SARS-CoV-2, we assessed the tropism and cytopathogenicity of the first Swedish isolate of SARS-CoV-2 in six different cell-lines of human origin and compared their growth characteristics to other globally isolated strains. Overall, majority of the strains showed high to moderate level of susceptibility in Caco2, Huh7, Calu 3 and 293FT cell-lines and in Caco2 cells the virus can achieve high titers in absence of any prominent cytopathic effect. The protein expression profile during SARS-CoV-2 infection revealed cell-type specific regulation of cellular pathways. Type-I interferon signaling was identified as the common dysregulated cellular response in Caco2, Calu-3 and Huh7 cells. Overall, cell-type specific variability was noted with respect to cytopathogenicity, susceptibility and cellular response to SARS-CoV-2. This study provides important clues regarding SARS-CoV-2 pathogenesis and can represent as a guide for future studies.
HostingRepositoryPRIDE
AnnounceDate2021-04-14
AnnouncementXMLSubmission_2021-04-14_00:36:55.591.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJIMMY RODRIGUEZ
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-22 08:39:28ID requested
12021-04-14 00:36:55announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Covid-19
submitter keyword: SARS-CoV-2, cellular tropism, cytopathogenicity, cell-lines, ACE-2, TMPRSS2, Type-I Intereferon, proteomics
Contact List
Akos Vegvari
contact affiliationDivision of Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
contact emailakos.vegvari@ki.se
lab head
JIMMY RODRIGUEZ
contact affiliationDivision of Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact emailesneider007@gmail.com
dataset submitter
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