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PXD023747 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDivergent evolutionary strategies for selective sex chromosome regulation in Drosophila
DescriptionThe dosage compensation complex (DCC) of Drosophila identifies its X chromosomal binding sites with exquisite selectivity. The principles that assure this vital targeting are known from the D. melanogaster model: DCC-intrinsic specificity of DNA binding, cooperativity with the CLAMP protein, and non-coding roX2 RNA transcribed from the X chromosome. We found that in D. virilis, a species separated from melanogaster by 40 million years of evolution, all principles are active, but contribute differently to X-specificity. In melanogaster, the DCC subunit MSL2 evolved intrinsic DNA-binding selectivity for rare PionX sites, which mark the X chromosome. In virilis, PionX sites are abundant and not X-enriched. Accordingly, MSL2 lacks specific recognition. Here, roX2 RNA plays a more instructive role, counteracting a non-productive interaction of CLAMP and modulating DCC binding selectivity. Remarkably, roX2 triggers a low-diffusion chromatin binding mode characteristic of DCC. Evidently, X-specific regulation is achieved by divergent evolution of similar components.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIgnasi Forne
SpeciesList scientific name: Drosophila virilis; NCBI TaxID: 7244;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-22 06:42:14ID requested
12021-09-14 08:20:53announced
Publication List
Villa R, Jagtap PKA, Thomae AW, Campos Sparr A, Forné I, Hennig J, Straub T, Becker PB, . Genes Dev, 35(13-14):1055-1070(2021) [pubmed]
Keyword List
submitter keyword: dosage compensation, evolution, MSL2, virilis, roX, long non-coding RNA, targeting
Contact List
Prof Peter B. Becker
contact affiliationMolecular Biology Dept, Biomedical Center, Ludwig-Maximilians-University Munich, Großhadernerstr. 9, 82152 Planegg-Martinsried (Germany)
contact emailpbecker@bmc.med.lmu.de
lab head
Ignasi Forne
contact affiliationBiomedical Center-LMU
contact emailignasi.forne@lrz.uni-muenchen.de
dataset submitter
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