PXD023747-1

PXD023747 is an original dataset announced via ProteomeXchange.

Title	Divergent evolutionary strategies for selective sex chromosome regulation in Drosophila
Description	The dosage compensation complex (DCC) of Drosophila identifies its X chromosomal binding sites with exquisite selectivity. The principles that assure this vital targeting are known from the D. melanogaster model: DCC-intrinsic specificity of DNA binding, cooperativity with the CLAMP protein, and non-coding roX2 RNA transcribed from the X chromosome. We found that in D. virilis, a species separated from melanogaster by 40 million years of evolution, all principles are active, but contribute differently to X-specificity. In melanogaster, the DCC subunit MSL2 evolved intrinsic DNA-binding selectivity for rare PionX sites, which mark the X chromosome. In virilis, PionX sites are abundant and not X-enriched. Accordingly, MSL2 lacks specific recognition. Here, roX2 RNA plays a more instructive role, counteracting a non-productive interaction of CLAMP and modulating DCC binding selectivity. Remarkably, roX2 triggers a low-diffusion chromatin binding mode characteristic of DCC. Evidently, X-specific regulation is achieved by divergent evolution of similar components.
HostingRepository	PRIDE
AnnounceDate	2021-09-14
AnnouncementXML	Submission_2021-09-14_08:20:52.429.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Drosophila virilis; NCBI TaxID: 7244;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-22 06:42:14	ID requested
⏵ 1	2021-09-14 08:20:53	announced

Villa R, Jagtap PKA, Thomae AW, Campos Sparr A, Forn, é I, Hennig J, Straub T, Becker PB, . Genes Dev, 35(13-14):1055-1070(2021) [pubmed]

submitter keyword: dosage compensation, evolution, MSL2, virilis, roX, long non-coding RNA, targeting

Prof Peter B. Becker
contact affiliation	Molecular Biology Dept, Biomedical Center, Ludwig-Maximilians-University Munich, Großhadernerstr. 9, 82152 Planegg-Martinsried (Germany)
contact email	pbecker@bmc.med.lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD023747

PRIDE project URI

• PRIDE
  1. PXD023747
     1. Label: PRIDE project
     2. Name: Divergent evolutionary strategies for selective sex chromosome regulation in Drosophila