PXD023509 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HyperLOPIT and temporal proteomic profiling of the response to lipopolysaccharide in the THP-1 human leukaemia cell line |
Description | The hyperLOPIT proteomics platform combines mass-spectrometry with state-of-the-art machine learning for simultaneous “mapping” of the steady-state subcellular location of thousands of proteins. Here, we use a synergistic approach and combine global proteome analysis with hyperLOPIT in a fully Bayesian framework to elucidate the spatio-temporal changes that occur during the pro-inflammatory response to lipopolysaccharide in the human monocytic leukaemia cell-line THP-1. We report cell-wide protein relocalisations upon LPS stimulation. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-16 |
AnnouncementXML | Submission_2022-02-16_14:54:46.427.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Claire Mulvey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-11 04:38:32 | ID requested | |
⏵ 1 | 2022-02-16 14:54:47 | announced | |
2 | 2023-05-14 14:13:32 | announced | 2023-05-14: Updated project metadata. |
Publication List
Mulvey CM, Breckels LM, Crook OM, Sanders DJ, Ribeiro ALR, Geladaki A, Christoforou A, Britov, š, ek NK, Hurrell T, Deery MJ, Gatto L, Smith AM, Lilley KS, Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line. Nat Commun, 12(1):5773(2021) [pubmed] |
Keyword List
submitter keyword: THP-1 cells, hyperLOPIT, LC-MS/MS, immune response |
Contact List
Kathryn Susan Lilley |
contact affiliation | University of Cambridge |
contact email | ksl23@cam.ac.uk |
lab head | |
Claire Mulvey |
contact affiliation | Cancer Research UK, Cambridge Institute, University of Cambridge |
contact email | claire.mulvey@cruk.cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD023509 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023509
- Label: PRIDE project
- Name: HyperLOPIT and temporal proteomic profiling of the response to lipopolysaccharide in the THP-1 human leukaemia cell line