PXD023509

PXD023509 is an original dataset announced via ProteomeXchange.

Title	HyperLOPIT and temporal proteomic profiling of the response to lipopolysaccharide in the THP-1 human leukaemia cell line
Description	The hyperLOPIT proteomics platform combines mass-spectrometry with state-of-the-art machine learning for simultaneous “mapping” of the steady-state subcellular location of thousands of proteins. Here, we use a synergistic approach and combine global proteome analysis with hyperLOPIT in a fully Bayesian framework to elucidate the spatio-temporal changes that occur during the pro-inflammatory response to lipopolysaccharide in the human monocytic leukaemia cell-line THP-1. We report cell-wide protein relocalisations upon LPS stimulation.
HostingRepository	PRIDE
AnnounceDate	2023-05-14
AnnouncementXML	Submission_2023-05-14_14:13:31.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ClaireMulvey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-11 04:38:32	ID requested
1	2022-02-16 14:54:47	announced
⏵ 2	2023-05-14 14:13:32	announced	2023-05-14: Updated project metadata.

Mulvey CM, Breckels LM, Crook OM, Sanders DJ, Ribeiro ALR, Geladaki A, Christoforou A, Britov, š, ek NK, Hurrell T, Deery MJ, Gatto L, Smith AM, Lilley KS, Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line. Nat Commun, 12(1):5773(2021) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: hyperLOPIT,THP-1 cells, immune response, LC-MS/MS

Kathryn SusanLilley
contact affiliation	University of Cambridge
contact email	ksl23@cam.ac.uk
lab head
ClaireMulvey
contact affiliation	Cancer Research UK, Cambridge Institute, University of Cambridge
contact email	claire.mulvey@cruk.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD023509

PRIDE project URI

• PRIDE
  1. PXD023509
     1. Label: PRIDE project
     2. Name: HyperLOPIT and temporal proteomic profiling of the response to lipopolysaccharide in the THP-1 human leukaemia cell line