PXD023444 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Intermittent bulk release of human cytomegalovirus |
Description | Human Cytomegalovirus (HCMV) significantly remodels host cell processes and membranes to enable effective production of virus progeny. Nascent capsids exit the nucleus and undergo a final morphogenesis process in the cytoplasm where they associate with tegument and membranes. During this process called secondary envelopment, fully assembled virions are formed which are wrapped inside of host vesicles. Both the origin of the receiving membrane as well as the dynamic organization of the envelopment and exocytosis process are not well understood. Enveloped virus particles can be both found individually wrapped in small vesicles but also as accumulations in multivesicular bodies (MVBs). While individually wrapped particles are believed to exit the cell by exocytosis, it is unclear if particles in MVBs ever leave the cell or are actually targeted for lysosomal degradation. Using a novel correlative light and electron microscopy workflow that allows three-dimensional imaging of whole cells, we present evidence that HCMV capsids can bud into MVBs, generating large intracellular accumulations of enveloped virions. Immunofluorescence and fusion assays suggest that these MVBs are positive for markers of the exosomal pathway. Time-lapse as well as functional live-cell imaging showed that MVBs fuse with the plasma membrane and release patch-like accumulations of virus particles. Our data suggests a so far undescribed envelopment and exocytosis pathway of HCMV, possibly involving the late-endosome/exosome pathway, leading to the intermittent bulk release of virus particles. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-23 |
AnnouncementXML | Submission_2022-06-23_04:27:59.395.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD023444 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Hannah Britt |
SpeciesList | scientific name: Human betaherpesvirus 5; NCBI TaxID: 10359; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-06 07:39:55 | ID requested | |
⏵ 1 | 2022-06-23 04:27:59 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human Cytomegalovirus, Integrative Microscopy, Cellular Egress, Morphogenesis |
Contact List
Konstantinos Thalassinos |
contact affiliation | 1) Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, United Kingdom 2) Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, United Kingdom |
contact email | k.thalassinos@ucl.ac.uk |
lab head | |
Hannah Britt |
contact affiliation | University College London |
contact email | h.britt@ucl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023444
- Label: PRIDE project
- Name: Intermittent bulk release of human cytomegalovirus