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PXD023361-1

PXD023361 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Cdk8 kinase module regulates Mediator-RNA polymerase II interaction
DescriptionWe use biochemically reconstituted transcription initiation components including the Mediator core and Cdk8 kinase modules (CKM) to investigate the function of the CKM in regulating the Mediator-RNA polymerase II interaction in a highly purified system. We use cross-linking coupled to mass spectrometry to map the interaction of the CKM and core Mediator, and in vitro phosphorylation assays followed by phosphopeptide enrichment coupled to mass spectrometry to identify Cdk8 phosphorylation targets in this context and biochemically dissect their functions. Finally, we investigate the function of phosphorylation by Cdk8 on transcription in vivo. Based on that, we propose a model to integrate the phosphorylation-dependent and -independent functions of the CKM in transcription initiation.
HostingRepositoryPRIDE
AnnounceDate2021-04-28
AnnouncementXMLSubmission_2021-04-28_10:27:03.750.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFanni Laura Bazso
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-04 01:48:22ID requested
12021-04-28 10:27:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: RNA polymerase II, transcription, Mediator, Cdk8
Contact List
Henning Urlaub
contact affiliationMax Planck Institute for Biophysical Chemistry, Göttingen,Germany and Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany
contact emailhenning.urlaub@mpibpc.mpg.de
lab head
Fanni Laura Bazso
contact affiliationMax Planck Institute for Biophysical Chemistry
contact emailfanni-laura.bazso@mpibpc.mpg.de
dataset submitter
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