PXD023123

PXD023123 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of human MV4-11 AML cells treated with Gilteritinib
Description	Internal tandem duplications (ITD) in the receptor tyrosine kinase FLT3 occur in 25% of acute myeloid leukemia (AML) patients and lead to constitutive activation of FLT3, driving leukemia cell survival and proliferation. Quizartinib, crenolanib and gilteritinib are second-generation FLT3 inhibitors (FLT3i) in phase III trials or clinical use for the targeted treatment of FLT3-ITD+ AML. However, they demonstrated only limited benefit and were not curative. A full understanding of cellular resistance factors contributing to this poor response is lacking. Here, we examined cell-autonomous pathways modulated by FLT3i using global translatome and phosphoproteome proteomics to identify non-genetic resistance mechanisms.
HostingRepository	PRIDE
AnnounceDate	2022-08-25
AnnouncementXML	Submission_2022-08-25_07:06:33.894.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastian Koschade
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; alkylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-14 06:44:45	ID requested
⏵ 1	2022-08-25 07:06:34	announced

Koschade SE, Klann K, Shaid S, Vick B, Stratmann JA, Th, ö, lken M, Meyer LM, Nguyen TD, Campe J, Moser LM, Hock S, Baker F, Meyer CT, Wempe F, Serve H, Ullrich E, Jeremias I, M, ü, nch C, Brandts CH, Translatome proteomics identifies autophagy as a resistance mechanism to on-target FLT3 inhibitors in acute myeloid leukemia. Leukemia, 36(10):2396-2407(2022) [pubmed]

submitter keyword: Human, AML, FLT3, LC-MSMS

Christian Münch
contact affiliation	Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany
contact email	ch.muench@em.uni-frankfurt.de
lab head
Sebastian Koschade
contact affiliation	Department of Medicine, Hematology/Oncology, University Hospital Frankfurt, Goethe University, Frankfurt, Germany & Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany
contact email	koschade@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD023123

PRIDE project URI

• PRIDE
  1. PXD023123
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of human MV4-11 AML cells treated with Gilteritinib