PXD023027-1

PXD023027 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of serum and cerebrospinal fluid samples of Guillain-Barré Syndrome patients
Description	To identify proteins that are expressed at high levels in samples of cerebrospinal fluid (CSF) from Guillain-Barré Syndrome (GBS) patients and determine whether such proteins are involved in the pathogenesis of this disease, liquid chromatography-tandem mass spectrometry was performed to determine the proteome of GBS patients, and multiple sclerosis and cerebral infarction patients were arranged for case groups. We detected the samples of neuromyelitis optica Spectrum disorders (NMOSD) patients at the same time, but the results were not included in this article. The discovery set featured 5 patients, while the validation set featured 20 patients in each of the three groups. For validation set, every 10 samples were divided into a group and mix detection was performed, and each group was tested twice. To eliminate the potential influence of blood-borne proteins entering the CSF via the blood-brain barrier, we performed proteomics analysis in paired serum and CSF samples. This allowed us to identify a profile of proteins that were expressed in the CSF but not the serum. Functional enrichment analysis was performed by FunRich. We used the STRING website, along with Cytoscape and Molecular Complex Detection, to investigate the protein–protein interaction (PPI) network. In total, 135 and 231 proteins from the discovery and validation sets of GBS patients, respectively, were CSF-specific. Of these, 99 proteins were detected in both sets of samples. Several proteins play a key role in the regulation of Ca2+, including calmodulin, chromogranin-A, et al. The PPI network featured 84 nodes and 238 interactions; one significant module and 8 hub genes were identified. Several protein pathways that were closely related to Ca2+ regulation were found to be significantly enriched in CSF samples from GBS patients. Our data indicate that the pathogenesis of GBS may be associated with an imbalance of Ca2+.
HostingRepository	iProX
AnnounceDate	2020-12-09
AnnouncementXML	Submission_2021-08-17_21:36:50.403.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruimin Ma
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-09 18:21:16	ID requested
⏵ 1	2021-08-17 21:36:50	announced
2	2021-08-18 01:24:47	announced	2021-08-18: Update information.
3	2023-02-21 18:33:13	announced	2021-08-18: Update information. 2023-02-22: Update publication information.

Dataset with its publication pending

submitter keyword: Guillain-Barré Syndrome,serum, cerebrospinal fluid, proteomics, calcium imbalance

Guojun Zhang
contact affiliation	Beijing Tiantan Hospital, Capital medical university
contact email	tiantandr@yeah.net
lab head
Ruimin Ma
contact affiliation	Beijing Tiantan Hospital, Capital medical university
contact email	mrmttyy@163.com
dataset submitter

iProX dataset URI