PXD022995

PXD022995 is an original dataset announced via ProteomeXchange.

Title	Ross Sea Surface Water Metaproteomics
Description	We sampled the microbial community at the sea ice edge in McMurdo Sound, Ross Sea at the same location (-77.62S, 165.41E) for four weeks (as described in Wu et al 2019, Nat. Comms.). We had four sampling dates corresponding to weeks 1 to 4: December 28 2014, January 6, 15, and 22 2015. Large volumes of water (150--250 L) were filtered from 1 m depth at the sea ice edge, and passed through three filters sequentially (3.0, 0.8, and 0.1 um, each 293 mm Supor filters). Filters with collected biomass were then placed in tubes with a sucrose-based preservative buffer (20 mM EDTA, 400 mM NaCl, 0.75 M sucrose, 50 mM Tris-HCl, pH 8.0) and stored at -80 C until sample processing. We extracted proteins after buffer exchange into a 3\% SDS solution as previously described Wu et al 2019, Nat. Comms.
HostingRepository	PRIDE
AnnounceDate	2021-09-07
AnnouncementXML	Submission_2021-09-07_04:09:20.560.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Scott McCain
SpeciesList	scientific name: marine plankton environmental sample; NCBI TaxID: 632957;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-08 06:43:19	ID requested
⏵ 1	2021-09-07 04:09:21	announced

McCain JSP, Tagliabue A, Susko E, Achterberg EP, Allen AE, Bertrand EM, Cellular costs underpin micronutrient limitation in phytoplankton. Sci Adv, 7(32):(2021) [pubmed]

submitter keyword: phytoplankton

Erin M. Bertrand
contact affiliation	Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada
contact email	erin.bertrand@dal.ca
lab head
Scott McCain
contact affiliation	Dalhousie University
contact email	j.scott.mccain@dal.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022995

PRIDE project URI

• PRIDE
  1. PXD022995
     1. Label: PRIDE project
     2. Name: Ross Sea Surface Water Metaproteomics