PXD022964 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A conserved cysteine acts as an endogenous antioxidant in the P-type plasma membrane H+-ATPase |
Description | Sulfur-containing amino acid residues function in antioxidative responses, which can be induced by the reactive oxygen species generated by excessive copper and hydrogen peroxide. In all Na+/K+, Ca2+, and H+ pumping P-type ATPases, a cysteine residue is present two residues upstream of the essential aspartate residue, which is obligatorily phosphorylated in each catalytic cycle. Despite its conservation, the function of this cysteine residue was hitherto unknown. In this study, we analyzed the function of the corresponding cysteine residue (Cys-327) in the autoinhibited plasma membrane H+-ATPase isoform 2 (AHA2) from Arabidopsis thaliana by mutagenesis and heterologous expression in a yeast host. Enzyme kinetics of alanine, serine, and leucine substitutions were identical to those of the wild-type pump but the sensitivity of the mutant pumps was increased towards copper and hydrogen peroxide. Additionally, the cysteine residue was prone to oxidation. These data suggest that Cys-327 functions as an endogenous antioxidant in the plasma membrane H+-ATPase, and possibly in other P-type ATPases as well. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-10 |
AnnouncementXML | Submission_2021-08-10_06:45:51.144.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sandra Maass |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | N-ethylmaleimide derivatized cysteine; monohydroxylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-07 03:10:32 | ID requested | |
1 | 2021-08-10 03:47:54 | announced | |
⏵ 2 | 2021-08-10 06:45:51 | announced | 2021-08-10: Updated project metadata. |
Publication List
Welle M, Pedersen JT, Ravnsborg T, Hayashi M, Maa, ß S, Becher D, Jensen ON, St, ö, hr C, Palmgren M, A conserved, buried cysteine near the P-site is accessible to cysteine modifications and increases ROS stability in the P-type plasma membrane H+-ATPase. Biochem J, 478(3):619-632(2021) [pubmed] |
10.1042/BCJ20200559; |
Keyword List
submitter keyword: conserved cysteine, endogenous antioxidant, proton pump |
Contact List
Dörte Becher |
contact affiliation | Department of Microbial Proteomics, Institute of Microbiology, University of Greifswald, Germany |
contact email | dbecher@uni-greifswald.de |
lab head | |
Sandra Maass |
contact affiliation | University of Greifswald, Department for Microbial Proteomics |
contact email | sandra.maass@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022964
- Label: PRIDE project
- Name: A conserved cysteine acts as an endogenous antioxidant in the P-type plasma membrane H+-ATPase