PXD022964

PXD022964 is an original dataset announced via ProteomeXchange.

Title	A conserved cysteine acts as an endogenous antioxidant in the P-type plasma membrane H+-ATPase
Description	Sulfur-containing amino acid residues function in antioxidative responses, which can be induced by the reactive oxygen species generated by excessive copper and hydrogen peroxide. In all Na+/K+, Ca2+, and H+ pumping P-type ATPases, a cysteine residue is present two residues upstream of the essential aspartate residue, which is obligatorily phosphorylated in each catalytic cycle. Despite its conservation, the function of this cysteine residue was hitherto unknown. In this study, we analyzed the function of the corresponding cysteine residue (Cys-327) in the autoinhibited plasma membrane H+-ATPase isoform 2 (AHA2) from Arabidopsis thaliana by mutagenesis and heterologous expression in a yeast host. Enzyme kinetics of alanine, serine, and leucine substitutions were identical to those of the wild-type pump but the sensitivity of the mutant pumps was increased towards copper and hydrogen peroxide. Additionally, the cysteine residue was prone to oxidation. These data suggest that Cys-327 functions as an endogenous antioxidant in the plasma membrane H+-ATPase, and possibly in other P-type ATPases as well.
HostingRepository	PRIDE
AnnounceDate	2021-08-10
AnnouncementXML	Submission_2021-08-10_06:45:51.144.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	N-ethylmaleimide derivatized cysteine; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 03:10:32	ID requested
1	2021-08-10 03:47:54	announced
⏵ 2	2021-08-10 06:45:51	announced	2021-08-10: Updated project metadata.

Welle M, Pedersen JT, Ravnsborg T, Hayashi M, Maa, ß S, Becher D, Jensen ON, St, ö, hr C, Palmgren M, A conserved, buried cysteine near the P-site is accessible to cysteine modifications and increases ROS stability in the P-type plasma membrane H+-ATPase. Biochem J, 478(3):619-632(2021) [pubmed]

10.1042/BCJ20200559;

submitter keyword: conserved cysteine, endogenous antioxidant, proton pump

Dörte Becher
contact affiliation	Department of Microbial Proteomics, Institute of Microbiology, University of Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022964
     1. Label: PRIDE project
     2. Name: A conserved cysteine acts as an endogenous antioxidant in the P-type plasma membrane H+-ATPase