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PXD022821-2

PXD022821 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe proteomic response of the coral symbiont Symbiodinium microadriaticum to thermal and nutrient stress
DescriptionAbstract The coral–dinoflagellate symbiosis is increasingly disrupted by global and local anthropogenic stressors. Coral bleaching is primarily a result of high sea surface temperatures, while eutrophication is associated with reef ecosystem degradation. Excess inorganic nitrogen relative to phosphate has been proposed to directly sensitise corals to thermal bleaching and accelerate reef decline. We assessed the proteomic response of the dinoflagellate coral symbiont Symbiodinium microadriaticum to elevated temperatures under multiple nutrient conditions by mass spectrometry. Elevated temperatures resulted in reductions of many chloroplast proteins, particularly light-harvesting complexes, with simultaneous increases in chaperone proteins. N:P imbalance had a larger effect on the proteome than temperature, but the biological processes and proteins responding to each stressor largely overlapped. The proteomes were highly similar at low N:P ratios but were strongly affected by phosphate starvation. High N:P ratios inhibited cell division, reflected by changes in proteins involved in protein translation. Imbalanced N:P did not increase sensitivity to high temperatures as measured by physiological means; however, imbalanced N:P strongly upregulated cell redox homeostasis proteins at high temperatures. As redox balance is critical during thermal bleaching, these data provide insight into the mechanisms of cellular responses to thermal and multiple stresses in the coral–dinoflagellate symbiosis.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:31.590.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterClinton Oakley
SpeciesList scientific name: Symbiodinium microadriaticum; NCBI TaxID: 2951;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-30 03:59:28ID requested
12023-01-30 19:12:54announced
22023-11-14 08:51:32announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: nutrient stress, thermal stress, coral reefs, coral bleaching,Symbiodinium
Contact List
Simon Keith Davy
contact affiliationSchool of Biological Sciences, Victoria University of Wellington, New Zealand
contact emailsimon.davy@vuw.ac.nz
lab head
Clinton Oakley
contact affiliationVictoria University of Wellington
contact emailclint.oakley@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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