PXD022773-1

PXD022773 is an original dataset announced via ProteomeXchange.

Title	GAK and PRKCD are positive regulators of Parkin-independent mitophagy
Description	Mitophagy plays a key cellular role for the programmed or damage induced removal of mitochondria, however, precisely how this process is orchestrated in response to different stimuli still requires investigation. In this work we have screened for regulators of PARKIN-independent mitophagy using an siRNA library targeting 200 proteins containing lipid interacting domains. We identify Cyclin-G associated kinase (GAK) and Protein Kinase C Delta (PRKCD) as novel regulators of PARKIN independent mitophagy. We demonstrate that the kinase activity of both GAK and PRKCD are required for efficient mitophagy in vitro, that PRKCD is present on mitochondria, and that PRKCD is required for ULK1/ATG13 recruitment to early autophagic structures. Importantly, we demonstrate in vivo relevance for both kinases in the regulation of basal mitophagy. Knockdown of GAK homologue (gakh-1) in C.elegans or PRKCD homologues in zebrafish led to significant inhibition of mitophagy, highlighting the evolutionary relevance of these kinases in mitophagy
HostingRepository	PRIDE
AnnounceDate	2021-09-29
AnnouncementXML	Submission_2021-09-29_01:27:55.967.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sachin Singh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-26 14:24:13	ID requested
⏵ 1	2021-09-29 01:27:56	announced

Dataset with its publication pending

submitter keyword: GAK, Cyclin G Associated Kinase, PRKCD, Protein Kinase C Delta, Mitophagy, DFP

Anne Simonsen
contact affiliation	Division of Biochemistry, Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Norway
contact email	anne.simonsen@medisin.uio.no
lab head
Sachin Singh
contact affiliation	Proteomics core facility, Olso University hospital, Norway
contact email	sachin.kumar.singh@rr-research.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022773
     1. Label: PRIDE project
     2. Name: GAK and PRKCD are positive regulators of Parkin-independent mitophagy