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PXD022773

PXD022773 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGAK and PRKCD are positive regulators of Parkin-independent mitophagy
DescriptionMitophagy plays a key cellular role for the programmed or damage induced removal of mitochondria, however, precisely how this process is orchestrated in response to different stimuli still requires investigation. In this work we have screened for regulators of PARKIN-independent mitophagy using an siRNA library targeting 200 proteins containing lipid interacting domains. We identify Cyclin-G associated kinase (GAK) and Protein Kinase C Delta (PRKCD) as novel regulators of PARKIN independent mitophagy. We demonstrate that the kinase activity of both GAK and PRKCD are required for efficient mitophagy in vitro, that PRKCD is present on mitochondria, and that PRKCD is required for ULK1/ATG13 recruitment to early autophagic structures. Importantly, we demonstrate in vivo relevance for both kinases in the regulation of basal mitophagy. Knockdown of GAK homologue (gakh-1) in C.elegans or PRKCD homologues in zebrafish led to significant inhibition of mitophagy, highlighting the evolutionary relevance of these kinases in mitophagy
HostingRepositoryPRIDE
AnnounceDate2021-09-29
AnnouncementXMLSubmission_2021-09-29_01:27:55.967.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSachin Singh
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-26 14:24:13ID requested
12021-09-29 01:27:56announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: GAK, Cyclin G Associated Kinase, PRKCD, Protein Kinase C Delta, Mitophagy, DFP
Contact List
Anne Simonsen
contact affiliationDivision of Biochemistry, Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Norway
contact emailanne.simonsen@medisin.uio.no
lab head
Sachin Singh
contact affiliationProteomics core facility, Olso University hospital, Norway
contact emailsachin.kumar.singh@rr-research.no
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
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