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PXD022722-1

PXD022722 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics of REPLICANT Perfusate Detects Changes in the Metastatic Lymph Node Microenvironment
Descriptionn breast cancer (BC), detecting low volumes of axillary lymph node (ALN) metastasis pre-operatively is difficult and novel biomarkers are needed. We recently showed that patient-derived ALNs can be sustained ex-vivo using normothermic perfusion. We now compare reactive (tumour-free; n = 5) and macrometastatic (containing tumour deposits >2mm; n = 4) ALNs by combining whole section multiplex immunofluorescence with TMT-labelled LC-MS/MS of the circulating perfusate. Macrometastases contained significantly fewer B- and T-cells (CD4+/CD8+/regulatory) than reactive nodes (p = 0.02). Similarly, pathway analysis of the perfusate proteome (119/1453 proteins significantly differentially expressed) showed that immune function was diminished in macrometastases in favour of ‘extracellular matrix degradation’; only ‘neutrophil degranulation’ was preserved. Qualitative comparison of the perfusate proteome to that of node-positive pancreatic and prostatic adenocarcinoma also highlighted ‘neutrophil degranulation’ as a contributing factor to nodal metastasis. Thus, metastasis-induced changes in the REPLICANT perfusate proteome are detectable, and could facilitate biomarker discovery.
HostingRepositoryPRIDE
AnnounceDate2021-01-05
AnnouncementXMLSubmission_2021-01-05_05:42:12.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Wright
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-25 03:27:24ID requested
12021-01-05 05:42:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cancer, LC-MSMS, TMT
Contact List
Jyoti Choudhary
contact affiliationThe Institute of Cancer Research
contact emailjyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliationThe Institute of Cancer Research
contact emailjames.wright@icr.ac.uk
dataset submitter
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Dataset FTP location
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