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PXD022711-1

PXD022711 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome-wide analysis of heat-stress in Pinus radiata somatic embryos reveals a combined response of sugar metabolism and translational regulation mechanisms
DescriptionRecent research has demonstrated that high temperatures during initial stages of conifer somatic embryogenesis can modulate the subsequent phases of the process, as well as the behaviour of the resulting plants ex vitro. In this sense, the development of high-throughput techniques has facilitated the study of the molecular response of plants to numerous stress factors. Proteomics offers a reliable image of the cell status and is known to be extremely susceptible to environmental temperature changes. As a result, in this study, the proteome of radiata pine somatic embryos was studies by LC-MS after the application of high temperatures during induction of embryonal masses (23 °C, 8 weeks, control, 40 °C, 4 h, 60 °C, 5 min). Then, the content of specific soluble sugars and sugar alcohols by HPLC was analysed. Results confirmed a decreased initiation rate of embryonal masses under high temperatures and a slight increase in the production of somatic embryos, as observed in previous studies. Besides, heat provoked a long-term readjustment of the protein synthesis machinery, by regulating translation and the composition of ribosomes. The levels of proteins related to methylation and posttranscriptional regulation mechanisms were also altered under high temperatures, coupled with higher contents of heat shock proteins and chaperones, transmembrane transport proteins, enzymes involved in the synthesis of fatty acids, specific compatible sugars (myo-inositol), and changes in the composition of cell-wall carbohydrates. These modifications occurred at the expense of the synthesis of molecules involved in oxidative stress responses, such as peroxidases, thiamine, phenolic compounds and some amino acids, by a decreased activity of the glycolytic pathway and reduced nitrogen assimilation.
HostingRepositoryPRIDE
AnnounceDate2021-04-09
AnnouncementXMLSubmission_2021-04-09_05:36:06.759.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVera Mendes
SpeciesList scientific name: Pinus radiata (Monterey pine); NCBI TaxID: 3347;
ModificationListS-carboxamidoethyl-L-cysteine
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-24 09:04:36ID requested
12021-04-09 05:36:07announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: carbohydrates, compatible solutes, heat shock proteins, high temperatures, methylation, proteomics, radiata pine, somatic embryogenesis.
Contact List
Paloma Moncaleán
contact affiliationDepartment of Forestry Science, NEIKER, Arkaute, Spain.
contact emailpmoncalean@neiker.eus
lab head
Vera Mendes
contact affiliationCenter for Neuroscience and Cell Biology, University of Coimbra
contact emailvera3m@gmail.com
dataset submitter
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Dataset FTP location
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