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PXD022701-1

PXD022701 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe ubiquitin ligase Ariadne-1 regulates NSF for neurotransmitter release
DescriptionThe aim of the present project was to identify putative substrates of Ariadne-1 (Ari-1), an essential E3 ubiquitin-ligase, in Drosophila heads. For that purpose we combined an in vivo ubiquitin biotinylation strategy termed bioUb with label free mass spectrometry analysis. In brief, BioUb strategy relies on the overexpression of a tagged ubiquitin that bears a 16 amino-acid long biotinylatable peptide, which can be biotinylated by the E.coli biotin holoenzyme synthetase enzyme (BirA) in neurons in vivo. Hence, ubiquitinated and therefore, biotinylated proteins can be enriched using avidin prior to MS analysis. We performed the same procedure in triplicate using flies expressing BioUb alone (control) and together with Ari-1. From the 1452 proteins quantified, 16 fulfilled the criteria established to be considered as putative Ari-1 substrates. Additionally, our analyses resulted in the detection of 150 peptides containing the characteristic GlyGly remnant of ubiquitin. Of those, 86 are novel ubiquitination sites not previously described in Drosophila.
HostingRepositoryPRIDE
AnnounceDate2021-02-09
AnnouncementXMLSubmission_2021-02-09_08:41:14.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNerea Osinalde
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListubiquitination signature dipeptidyl lysine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-24 07:52:10ID requested
12021-02-09 08:41:14announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Ariadne-1, Drosophila, E3 ubiquitin ligase, neurotransmitter release, NSF, synapse, ubiquitination
Contact List
Ugo Mayor
contact affiliationIkerbasque Researcher, Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, UPV/EHU, Leioa 48940, Bizkaia, Spain
contact emailugo.mayor@ehu.eus
lab head
Nerea Osinalde
contact affiliationTeacher/Researcher
contact emailnerea.osinalde@ehu.eus
dataset submitter
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Dataset FTP location
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