PXD022701

PXD022701 is an original dataset announced via ProteomeXchange.

Title	The ubiquitin ligase Ariadne-1 regulates NSF for neurotransmitter release
Description	The aim of the present project was to identify putative substrates of Ariadne-1 (Ari-1), an essential E3 ubiquitin-ligase, in Drosophila heads. For that purpose we combined an in vivo ubiquitin biotinylation strategy termed bioUb with label free mass spectrometry analysis. In brief, BioUb strategy relies on the overexpression of a tagged ubiquitin that bears a 16 amino-acid long biotinylatable peptide, which can be biotinylated by the E.coli biotin holoenzyme synthetase enzyme (BirA) in neurons in vivo. Hence, ubiquitinated and therefore, biotinylated proteins can be enriched using avidin prior to MS analysis. We performed the same procedure in triplicate using flies expressing BioUb alone (control) and together with Ari-1. From the 1452 proteins quantified, 16 fulfilled the criteria established to be considered as putative Ari-1 substrates. Additionally, our analyses resulted in the detection of 150 peptides containing the characteristic GlyGly remnant of ubiquitin. Of those, 86 are novel ubiquitination sites not previously described in Drosophila.
HostingRepository	PRIDE
AnnounceDate	2021-02-09
AnnouncementXML	Submission_2021-02-09_08:41:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nerea Osinalde
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-24 07:52:10	ID requested
⏵ 1	2021-02-09 08:41:14	announced

Dataset with its publication pending

submitter keyword: Ariadne-1, Drosophila, E3 ubiquitin ligase, neurotransmitter release, NSF, synapse, ubiquitination

Ugo Mayor
contact affiliation	Ikerbasque Researcher, Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, UPV/EHU, Leioa 48940, Bizkaia, Spain
contact email	ugo.mayor@ehu.eus
lab head
Nerea Osinalde
contact affiliation	Teacher/Researcher
contact email	nerea.osinalde@ehu.eus
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022701

PRIDE project URI

• PRIDE
  1. PXD022701
     1. Label: PRIDE project
     2. Name: The ubiquitin ligase Ariadne-1 regulates NSF for neurotransmitter release