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PXD022676-1

PXD022676 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis
DescriptionUridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular network connecting several translational repressors/decapping activators. URT1 directly interacts with DECAPPING 5 (DCP5), the Arabidopsis ortholog of human LSM14 and yeast Scd6, and this interaction connects URT1 to additional decay factors like DDX6/Dhh1-like RNA helicases. Nanopore direct RNA sequencing reveals a global role of URT1 in shaping poly(A) tail length, notably by preventing the accumulation of excessively deadenylated mRNAs. Based on in vitro and in planta data, we propose a model that explains how URT1 could reduce the accumulation of oligo(A)-tailed mRNAs both by favoring their degradation and because 3’ terminal uridines intrinsically hinder deadenylation. Importantly, preventing the accumulation of excessively deadenylated mRNAs avoids the biogenesis of illegitimate siRNAs that silence endogenous mRNAs and perturb Arabidopsis growth and development.
HostingRepositoryPRIDE
AnnounceDate2020-12-01
AnnouncementXMLSubmission_2020-12-01_00:17:11.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD022676
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLauriane Kuhn
SpeciesList scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-23 09:32:10ID requested
12020-12-01 00:17:11announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Uridylation, TUTase, RNA degradation, decapping, deadenylation, Arabidopsis, DCP5, GIGYF, Nanopore DRS
Contact List
Dominique Gagliardi
contact affiliation1Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 rue Zimmer, 67000 Strasbourg, France.
contact emaildominique.gagliardi@ibmp-cnrs.unistra.fr
lab head
Lauriane Kuhn
contact affiliationCNRS, IBMC, FRC1589
contact emaill.kuhn@ibmc-cnrs.unistra.fr
dataset submitter
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Dataset FTP location
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