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PXD022470

PXD022470 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA tailored phosphoaspartate probe unravels CprR as a response regulator in Pseudomonas aeruginosa interkingdom signaling
DescriptionPseudomonas aeruginosa is a difficult-to-treat Gram-negative bacterial pathogen causing life-threatening infections. Adaptive resistance (AR) to cationic peptide antibiotics such as polymyxin B impairs the therapeutic success. This self-protection is mediated by two component systems consisting of a membrane-bound histidine kinase and an intracellular response regulator (RR). As phosphorylation of the key RR aspartate residue is transient during signaling and hydrolytically unstable, the study of these systems is challenging. Therefore, we applied a tailored reverse polarity chemical proteomic strategy to capture this transient modification and read-out RR phosphorylation in complex proteomes using a nucleophilic probe. Analysis of Bacillus subtilis and P. aeruginosa proteomes revealed the detection of multiple phosphoaspartate sites, which closely resembled the conserved RR sequence motif. With this validated strategy we dissected the signaling of dynorphin A, a human peptide stress hormone, which is sensed by P. aeruginosa to prepare AR. Intriguingly, our methodology combined with parallel reaction monitoring identified CprR as an unprecedented RR in dynorphin A interkingdom signaling.
HostingRepositoryPanoramaPublic
AnnounceDate2021-03-14
AnnouncementXMLSubmission_2021-03-14_20:27:05.484.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterPatrick Allihn
SpeciesList scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationListCarbamidomethyl
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-11 06:25:46ID requested
12021-03-14 20:27:05announced
Publication List
Allihn PWA, Hackl MW, Ludwig C, Hacker SM, Sieber SA. A tailored phosphoaspartate probe unravels CprR as a response regulator in Pseudomonas aeruginosa interkingdom signaling. Chem Sci [Internet]. 2021
Available from: http://dx.doi.org/10.1039/D0SC06226J
Keyword List
submitter keyword: Activity-based protein profiling, isotopically labeled tags, phosphorylation, response regulator, parallel reaction monitoring, adaptive resistance, antimicrobial peptide, P. aeruginosa
Contact List
Stephan Sieber
contact affiliationTechnical University of Munich
contact emailstephan.sieber@tum.de
lab head
Patrick Allihn
contact affiliationTechnical University of Munich
contact emailpatrick.allihn@tum.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI