PXD022470-2
PXD022470 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A tailored phosphoaspartate probe unravels CprR as a response regulator in Pseudomonas aeruginosa interkingdom signaling |
Description | Pseudomonas aeruginosa is a difficult-to-treat Gram-negative bacterial pathogen causing life-threatening infections. Adaptive resistance (AR) to cationic peptide antibiotics such as polymyxin B impairs the therapeutic success. This self-protection is mediated by two component systems consisting of a membrane-bound histidine kinase and an intracellular response regulator (RR). As phosphorylation of the key RR aspartate residue is transient during signaling and hydrolytically unstable, the study of these systems is challenging. Therefore, we applied a tailored reverse polarity chemical proteomic strategy to capture this transient modification and read-out RR phosphorylation in complex proteomes using a nucleophilic probe. Analysis of Bacillus subtilis and P. aeruginosa proteomes revealed the detection of multiple phosphoaspartate sites, which closely resembled the conserved RR sequence motif. With this validated strategy we dissected the signaling of dynorphin A, a human peptide stress hormone, which is sensed by P. aeruginosa to prepare AR. Intriguingly, our methodology combined with parallel reaction monitoring identified CprR as an unprecedented RR in dynorphin A interkingdom signaling. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2021-08-19 |
AnnouncementXML | Submission_2021-08-19_09:05:51.408.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | supported by repository but incomplete data and/or metadata |
PrimarySubmitter | Patrick Allihn |
SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
ModificationList | Carbamidomethyl |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-11-11 06:25:46 | ID requested | |
1 | 2021-03-14 20:27:05 | announced | |
⏵ 2 | 2021-08-19 09:05:52 | announced | : Added PubMed Id |
Publication List
Allihn PWA, Hackl MW, Ludwig C, Hacker SM, Sieber SA, interkingdom signaling. Chem Sci, 12(13):4763-4770(2021) [pubmed] |
Keyword List
submitter keyword: Activity-based protein profiling, isotopically labeled tags, phosphorylation, response regulator, parallel reaction monitoring, adaptive resistance, antimicrobial peptide, P. aeruginosa |
Contact List
Stephan Sieber | |
---|---|
contact affiliation | Technical University of Munich |
contact email | stephan.sieber@tum.de |
lab head | |
Patrick Allihn | |
contact affiliation | Technical University of Munich |
contact email | patrick.allihn@tum.de |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |