PXD022458

PXD022458 is an original dataset announced via ProteomeXchange.

Title	Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis
Description	Toxoplasma gondii is an obligate intracellular parasite that can cause severe toxoplasmosis in humans and animals. Although T. gondii infection can disrupt the host's transcription, translation, metabolism and cause kidney damage, the mechanism remains largely unknown.By using a label-free proteomics quantitative method, we sequenced the urine proteome of female BALB/c mice at different phases of toxoplasmosis. A total of 1,802 proteins were obtained and proteome profiling of difference that easily distinguished between acutely infected groups and other groups was found. In this study, 169 and 47 differentially expressed proteins (DEPs) were found during the acute and chronic infection phases, respectively. Gene Ontology analysis showed that a large number of the these DEPs were associated with biological binding activity and single-organism process. KEGG pathway enrichment analysis showed that the majority of DEPs were involved in disease-related and metabolic pathways. Collectively, we have conducted in-depth exploration of mouse urine protein and revealed that urine protein variations were affected by the disease development process. This study provides new data on protein variations in urine of mice infected with T. gondii and can further understand the pathogenesis of mice. This research will help in the finding a key differential proteins, and provide effective data support for finding new treatment methods of toxoplasmosis.
HostingRepository	iProX
AnnounceDate	2020-11-10
AnnouncementXML	Submission_2023-03-02_18:38:31.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chunxue Zhou
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Mus ; NCBI TaxID: 10088;
ModificationList	oxidation
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-10 16:44:41	ID requested
1	2021-03-30 23:13:31	announced
⏵ 2	2023-03-02 18:38:32	announced	2023-03-03: Update publication information.

Cui LL, Zhou CX, Han B, Wang SS, Li SY, Xie SC, Zhou DH, Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis. Parasit Vectors, 14(1):211(2021) [pubmed]

submitter keyword: Toxoplasma gondii, urine, proteomics, biomarkers

Chunxue Zhou
contact affiliation	Shandong University
contact email	zhouchunxue23@163.com
lab head
Chunxue Zhou
contact affiliation	Shandong University
contact email	zhouchunxue23@163.com
dataset submitter

iProX dataset URI