Update publication information. Toxoplasma gondii is an obligate intracellular parasite that can cause severe toxoplasmosis in humans and animals. Although T. gondii infection can disrupt the host's transcription, translation, metabolism and cause kidney damage, the mechanism remains largely unknown.By using a label-free proteomics quantitative method, we sequenced the urine proteome of female BALB/c mice at different phases of toxoplasmosis. A total of 1,802 proteins were obtained and proteome profiling of difference that easily distinguished between acutely infected groups and other groups was found. In this study, 169 and 47 differentially expressed proteins (DEPs) were found during the acute and chronic infection phases, respectively. Gene Ontology analysis showed that a large number of the these DEPs were associated with biological binding activity and single-organism process. KEGG pathway enrichment analysis showed that the majority of DEPs were involved in disease-related and metabolic pathways. Collectively, we have conducted in-depth exploration of mouse urine protein and revealed that urine protein variations were affected by the disease development process. This study provides new data on protein variations in urine of mice infected with T. gondii and can further understand the pathogenesis of mice. This research will help in the finding a key differential proteins, and provide effective data support for finding new treatment methods of toxoplasmosis.