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PXD022432-1

PXD022432 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNucleolin rescues TDP-43 toxicity in yeast and human cell models
DescriptionTDP-43 is a nuclear protein involved in pivotal processes, extensively studied for its implication in neurodegenerative disorders. TDP-43 cytosolic inclusions are a common neuropathologic hallmark in amyotrophic lateral sclerosis (ALS) and related diseases, and it is now established that TDP-43 misfolding and aggregation play a key role in their etiopathology. TDP-43 neurotoxic mechanisms are not yet clarified, but the identification of proteins able to modulate TDP-43-mediated damage may be promising therapeutic targets for TDP-43 proteinopathies. Here we show by the use of refined yeast models that the nucleolar protein nucleolin (NCL) acts as a potent suppressor of TDP-43 toxicity, restoring cell viability. We provide evidence that NCL co-expression is able to alleviate TDP-43-induced damage also in human cells, further supporting its beneficial effects in a more consistent pathophysiological context. Presented data suggest that NCL could promote TDP-43 nuclear retention, reducing the formation of toxic cytosolic TDP-43 aggregates.
HostingRepositoryPRIDE
AnnounceDate2021-03-26
AnnouncementXMLSubmission_2021-03-25_23:10:52.978.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRoberto Stella
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-09 03:24:50ID requested
12021-03-25 23:10:53announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Neurodegenerative disorders, TDP-43 proteinopathies, ALS, FTD, nucleolin, nucleo-cytoplasmic transport, TMT, LC-HRMS/MS
Contact List
Alessandro Bertoli
contact affiliationDepartment of Biomedical Sciences, University of Padova, Italy CNR-Neuroscience Institute, Italy Padova Neuroscience center, University of Padova, Italy
contact emailalessandro.bertoli@unipd.it
lab head
Roberto Stella
contact affiliationIstituto Zooprofilattico Sperimentale delle Venezie
contact emailrstella@izsvenezie.it
dataset submitter
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Dataset FTP location
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