PXD022432

PXD022432 is an original dataset announced via ProteomeXchange.

Title	Nucleolin rescues TDP-43 toxicity in yeast and human cell models
Description	TDP-43 is a nuclear protein involved in pivotal processes, extensively studied for its implication in neurodegenerative disorders. TDP-43 cytosolic inclusions are a common neuropathologic hallmark in amyotrophic lateral sclerosis (ALS) and related diseases, and it is now established that TDP-43 misfolding and aggregation play a key role in their etiopathology. TDP-43 neurotoxic mechanisms are not yet clarified, but the identification of proteins able to modulate TDP-43-mediated damage may be promising therapeutic targets for TDP-43 proteinopathies. Here we show by the use of refined yeast models that the nucleolar protein nucleolin (NCL) acts as a potent suppressor of TDP-43 toxicity, restoring cell viability. We provide evidence that NCL co-expression is able to alleviate TDP-43-induced damage also in human cells, further supporting its beneficial effects in a more consistent pathophysiological context. Presented data suggest that NCL could promote TDP-43 nuclear retention, reducing the formation of toxic cytosolic TDP-43 aggregates.
HostingRepository	PRIDE
AnnounceDate	2021-03-26
AnnouncementXML	Submission_2021-03-25_23:10:52.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberto Stella
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-09 03:24:50	ID requested
⏵ 1	2021-03-25 23:10:53	announced

Dataset with its publication pending

submitter keyword: Neurodegenerative disorders, TDP-43 proteinopathies, ALS, FTD, nucleolin, nucleo-cytoplasmic transport, TMT, LC-HRMS/MS

Alessandro Bertoli
contact affiliation	Department of Biomedical Sciences, University of Padova, Italy CNR-Neuroscience Institute, Italy Padova Neuroscience center, University of Padova, Italy
contact email	alessandro.bertoli@unipd.it
lab head
Roberto Stella
contact affiliation	Istituto Zooprofilattico Sperimentale delle Venezie
contact email	rstella@izsvenezie.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD022432

PRIDE project URI

• PRIDE
  1. PXD022432
     1. Label: PRIDE project
     2. Name: Nucleolin rescues TDP-43 toxicity in yeast and human cell models