PXD022366 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Profound Immune Alterations in the Murine CML Bone Marrow Microenvironment Revealed by Single Cell Ph- Macrophage Profiling and Secretory Proteomics |
Description | Macrophages are cells of the innate immune system fundamental to support normal haematopoiesis, fight infection, anti-cancer immunity and tumour progression. However, the function of macrophages in myeloid leukaemias remain largely unknown, due to difficulties in isolating non-transformed cells from the malignant ones. Here we use a state-of-the-art chimeric mouse of chronic myeloid leukaemia (CML) to study in the impact of the dysregulated bone marrow (BM) microenvironment on bystander macrophages. Utilising single cell RNA sequencing (scRNA seq) of Philadelphia chromosome (Ph) negative macrophages and secretome proteomics of murine c-kit+ stem/progenitor cells we have uncovered that macrophages exposed to a CML environment are altered transcriptionally and have reduced phagocytic function |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-23 |
AnnouncementXML | Submission_2023-12-23_03:21:05.960.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sara Zanivan |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-05 00:34:51 | ID requested | |
⏵ 1 | 2023-12-23 03:21:06 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: macrophage,Secretome, leukaemia |
Contact List
Sara Zanivan |
contact affiliation | Prof. Sara Zanivan CRUK Beatson Institute Switchback road, Glasgow, UK G611BD |
contact email | s.zanivan@beatson.gla.ac.uk |
lab head | |
Sara Zanivan |
contact affiliation | Tumour Microenvironment & Proteomics |
contact email | s.zanivan@beatson.gla.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022366
- Label: PRIDE project
- Name: Profound Immune Alterations in the Murine CML Bone Marrow Microenvironment Revealed by Single Cell Ph- Macrophage Profiling and Secretory Proteomics