PXD022366

PXD022366 is an original dataset announced via ProteomeXchange.

Title	Profound Immune Alterations in the Murine CML Bone Marrow Microenvironment Revealed by Single Cell Ph- Macrophage Profiling and Secretory Proteomics
Description	Macrophages are cells of the innate immune system fundamental to support normal haematopoiesis, fight infection, anti-cancer immunity and tumour progression. However, the function of macrophages in myeloid leukaemias remain largely unknown, due to difficulties in isolating non-transformed cells from the malignant ones. Here we use a state-of-the-art chimeric mouse of chronic myeloid leukaemia (CML) to study in the impact of the dysregulated bone marrow (BM) microenvironment on bystander macrophages. Utilising single cell RNA sequencing (scRNA seq) of Philadelphia chromosome (Ph) negative macrophages and secretome proteomics of murine c-kit+ stem/progenitor cells we have uncovered that macrophages exposed to a CML environment are altered transcriptionally and have reduced phagocytic function
HostingRepository	PRIDE
AnnounceDate	2023-12-23
AnnouncementXML	Submission_2023-12-23_03:21:05.960.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Zanivan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-05 00:34:51	ID requested
⏵ 1	2023-12-23 03:21:06	announced

Dataset with its publication pending

submitter keyword: macrophage,Secretome, leukaemia

Sara Zanivan
contact affiliation	Prof. Sara Zanivan CRUK Beatson Institute Switchback road, Glasgow, UK G611BD
contact email	s.zanivan@beatson.gla.ac.uk
lab head
Sara Zanivan
contact affiliation	Tumour Microenvironment & Proteomics
contact email	s.zanivan@beatson.gla.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022366
     1. Label: PRIDE project
     2. Name: Profound Immune Alterations in the Murine CML Bone Marrow Microenvironment Revealed by Single Cell Ph- Macrophage Profiling and Secretory Proteomics