PXD022317

PXD022317 is an original dataset announced via ProteomeXchange.

Title	Global lysine acetylation and 2-hydroxyisobutyrylation reveal the metabolism conversion mechanism in Giardia lamblia
Description	Giardia lamblia (G. lamblia) disease is a zoonosis with a-infection rate affecting the general population of the world. Despite the constant possibility of damage due to their own metabolism, G. lamblia have survived and evolved to adapt to various environments. However, research on energy-metabolism conversion in G. lamblia is limited. This study aimed to reveal the dynamic metabolism-conversion mechanism in G. lamblia under sugar starvation by detecting global lysine acetylation and 2-hydroxyisobutyrylation sites combined with quantitative proteome analyses. A total of 2999 acetylation sites on 956 proteins and 7894 2-hydroxyisobutyryl sites on 1546 proteins were quantified under sugar starvation. Integrated Kac and Khib data revealed that modified proteins were associated with arginine biosynthesis, glycolysis/gluconeogenesis, and alanine, aspartate, and glutamate metabolism. These findings suggested that lysine acetylation and 2-hydroxyisobutyrylation were ubiquitous and provided deep insight into the metabolism-conversion mechanism in G. lamblia under sugar starvation. Overall, these results can help understand the biology of G. lamblia infections and reveal the evolution rule from prokaryote to eukaryote.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_13:46:58.227.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenhe Zhu
SpeciesList	scientific name: Giardia lamblia P15; NCBI TaxID: 658858;
ModificationList	acetylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-03 05:32:28	ID requested
⏵ 1	2021-03-04 13:46:58	announced

Zhu W, Jiang X, Sun H, Li Y, Shi W, Zheng M, Liu D, Ma A, Feng X, Global Lysine Acetylation and 2-Hydroxyisobutyrylation Profiling Reveals the Metabolism Conversion Mechanism in Giardia lamblia. Mol Cell Proteomics, 20():100043(2021) [pubmed]

submitter keyword: Giardia lamblia

acetylation

2-hydroxyisobutyrylation

Metabolism conversion.

Xianmin Feng
contact affiliation	Jilin Medical University
contact email	fengxianmin28@163.com
lab head
Wenhe Zhu
contact affiliation	Jilin Medical University
contact email	huolizwh@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022317
     1. Label: PRIDE project
     2. Name: Global lysine acetylation and 2-hydroxyisobutyrylation reveal the metabolism conversion mechanism in Giardia lamblia