PXD022313 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Simple and efficient micro-SPE tip-based sample preparation workflow to enable sensitive proteomic profiling of limited samples |
Description | In-depth LC-MS-based proteomic profiling of limited samples has been problematic due, in large part, to the inefficiency of sample preparation and attendant sample losses. To address this issue, we developed On-Micro Solid-phase Extraction Tip-based (OmSET) sample preparation for limited biological samples. OmSET is simple, efficient, reproducible, and scalable, and is a widely accessible method for processing ~200 to 10,000 cells. The developed method benefits from minimal sample processing volumes (1-3 μL) and conducting all sample processing steps on-membrane within a single microreactor. Here, we assessed the feasibility of using micro-SPE tips for nanogram-level amounts of tryptic peptides, minimized the number of required sample handling steps and reduced the hands-on time, and evaluated the capability of OmSET for quantitative analysis of low numbers of human monocytes. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-23 |
AnnouncementXML | Submission_2021-02-22_23:11:57.533.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | James Kostas |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-03 05:30:23 | ID requested | |
⏵ 1 | 2021-02-22 23:11:57 | announced | |
2 | 2021-02-26 02:08:55 | announced | 2021-02-26: Updated publication reference for PubMed record(s): 33625864. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: sample preparation, micro-SPE tip, StageTip, limited sample, OmSET, on-micro solid-phase extraction tip |
Contact List
Alexander R. Ivanov |
contact affiliation | Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Ave., Boston, Massachusetts 02115, United States |
contact email | a.ivanov@northeastern.edu |
lab head | |
James Kostas |
contact affiliation | Department of Chemistry and Chemical Biology, Northeastern University |
contact email | kostas.j@northeastern.edu |
dataset submitter | |
Full Dataset Link List
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022313
- Label: PRIDE project
- Name: Simple and efficient micro-SPE tip-based sample preparation workflow to enable sensitive proteomic profiling of limited samples