PXD022313-1

PXD022313 is an original dataset announced via ProteomeXchange.

Title	Simple and efficient micro-SPE tip-based sample preparation workflow to enable sensitive proteomic profiling of limited samples
Description	In-depth LC-MS-based proteomic profiling of limited samples has been problematic due, in large part, to the inefficiency of sample preparation and attendant sample losses. To address this issue, we developed On-Micro Solid-phase Extraction Tip-based (OmSET) sample preparation for limited biological samples. OmSET is simple, efficient, reproducible, and scalable, and is a widely accessible method for processing ~200 to 10,000 cells. The developed method benefits from minimal sample processing volumes (1-3 μL) and conducting all sample processing steps on-membrane within a single microreactor. Here, we assessed the feasibility of using micro-SPE tips for nanogram-level amounts of tryptic peptides, minimized the number of required sample handling steps and reduced the hands-on time, and evaluated the capability of OmSET for quantitative analysis of low numbers of human monocytes.
HostingRepository	PRIDE
AnnounceDate	2021-02-23
AnnouncementXML	Submission_2021-02-22_23:11:57.533.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Kostas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-03 05:30:23	ID requested
⏵ 1	2021-02-22 23:11:57	announced
2	2021-02-26 02:08:55	announced	2021-02-26: Updated publication reference for PubMed record(s): 33625864.

Dataset with its publication pending

submitter keyword: sample preparation, micro-SPE tip, StageTip, limited sample, OmSET, on-micro solid-phase extraction tip

Alexander R. Ivanov
contact affiliation	Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Ave., Boston, Massachusetts 02115, United States
contact email	a.ivanov@northeastern.edu
lab head
James Kostas
contact affiliation	Department of Chemistry and Chemical Biology, Northeastern University
contact email	kostas.j@northeastern.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022313

PRIDE project URI

• PRIDE
  1. PXD022313
     1. Label: PRIDE project
     2. Name: Simple and efficient micro-SPE tip-based sample preparation workflow to enable sensitive proteomic profiling of limited samples