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PXD022313

PXD022313 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSimple and efficient micro-SPE tip-based sample preparation workflow to enable sensitive proteomic profiling of limited samples
DescriptionIn-depth LC-MS-based proteomic profiling of limited samples has been problematic due, in large part, to the inefficiency of sample preparation and attendant sample losses. To address this issue, we developed On-Micro Solid-phase Extraction Tip-based (OmSET) sample preparation for limited biological samples. OmSET is simple, efficient, reproducible, and scalable, and is a widely accessible method for processing ~200 to 10,000 cells. The developed method benefits from minimal sample processing volumes (1-3 μL) and conducting all sample processing steps on-membrane within a single microreactor. Here, we assessed the feasibility of using micro-SPE tips for nanogram-level amounts of tryptic peptides, minimized the number of required sample handling steps and reduced the hands-on time, and evaluated the capability of OmSET for quantitative analysis of low numbers of human monocytes.
HostingRepositoryPRIDE
AnnounceDate2021-02-23
AnnouncementXMLSubmission_2021-02-26_02:08:54.869.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Kostas
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Q Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-03 05:30:23ID requested
12021-02-22 23:11:57announced
22021-02-26 02:08:55announced2021-02-26: Updated publication reference for PubMed record(s): 33625864.
Publication List
Kostas JC, Greguš M, Schejbal J, Ray S, Ivanov AR, Simple and Efficient Microsolid-Phase Extraction Tip-Based Sample Preparation Workflow to Enable Sensitive Proteomic Profiling of Limited Samples (200 to 10,000 Cells). J Proteome Res, 20(3):1676-1688(2021) [pubmed]
Keyword List
submitter keyword: sample preparation, micro-SPE tip, StageTip, limited sample, OmSET, on-micro solid-phase extraction tip
Contact List
Alexander R. Ivanov
contact affiliationBarnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Ave., Boston, Massachusetts 02115, United States
contact emaila.ivanov@northeastern.edu
lab head
James Kostas
contact affiliationDepartment of Chemistry and Chemical Biology, Northeastern University
contact emailkostas.j@northeastern.edu
dataset submitter
Full Dataset Link List
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