PXD022313 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Simple and efficient micro-SPE tip-based sample preparation workflow to enable sensitive proteomic profiling of limited samples |
Description | In-depth LC-MS-based proteomic profiling of limited samples has been problematic due, in large part, to the inefficiency of sample preparation and attendant sample losses. To address this issue, we developed On-Micro Solid-phase Extraction Tip-based (OmSET) sample preparation for limited biological samples. OmSET is simple, efficient, reproducible, and scalable, and is a widely accessible method for processing ~200 to 10,000 cells. The developed method benefits from minimal sample processing volumes (1-3 μL) and conducting all sample processing steps on-membrane within a single microreactor. Here, we assessed the feasibility of using micro-SPE tips for nanogram-level amounts of tryptic peptides, minimized the number of required sample handling steps and reduced the hands-on time, and evaluated the capability of OmSET for quantitative analysis of low numbers of human monocytes. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-23 |
AnnouncementXML | Submission_2021-02-26_02:08:54.869.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | James Kostas |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-03 05:30:23 | ID requested | |
1 | 2021-02-22 23:11:57 | announced | |
⏵ 2 | 2021-02-26 02:08:55 | announced | 2021-02-26: Updated publication reference for PubMed record(s): 33625864. |
Publication List
Kostas JC, Gregu, š M, Schejbal J, Ray S, Ivanov AR, Simple and Efficient Microsolid-Phase Extraction Tip-Based Sample Preparation Workflow to Enable Sensitive Proteomic Profiling of Limited Samples (200 to 10,000 Cells). J Proteome Res, 20(3):1676-1688(2021) [pubmed] |
Keyword List
submitter keyword: sample preparation, micro-SPE tip, StageTip, limited sample, OmSET, on-micro solid-phase extraction tip |
Contact List
Alexander R. Ivanov |
contact affiliation | Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Ave., Boston, Massachusetts 02115, United States |
contact email | a.ivanov@northeastern.edu |
lab head | |
James Kostas |
contact affiliation | Department of Chemistry and Chemical Biology, Northeastern University |
contact email | kostas.j@northeastern.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022313 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022313
- Label: PRIDE project
- Name: Simple and efficient micro-SPE tip-based sample preparation workflow to enable sensitive proteomic profiling of limited samples