PXD022252-1

PXD022252 is an original dataset announced via ProteomeXchange.

Title	Dual DNA/protein tagging of open chromatin unveils dynamics of epigenomic landscapes in leukemia
Description	The architecture of chromatin specifies eukaryotic cell identity by controlling transcription factor access to sites of gene regulation. Here we describe a dual transposase/peroxidase approach, integrative DNA And Protein Tagging (iDAPT), which detects both DNA (iDAPT-seq) and protein (iDAPT-MS) associated with accessible regions of chromatin. In addition to direct identification of bound transcription factors, iDAPT enables the inference of their gene regulatory networks, protein interactors, and regulation of chromatin accessibility. We applied iDAPT to profile the epigenomic consequences of granulocytic differentiation of acute promyelocytic leukemia, yielding previously undescribed mechanistic insights with potential therapeutic implications. Our findings demonstrate the power of iDAPT as a discovery platform for both the dynamic epigenomic landscapes and their transcription factor components associated with biological phenomena and disease.
HostingRepository	PRIDE
AnnounceDate	2020-11-02
AnnouncementXML	Submission_2020-11-01_22:47:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-29 08:28:39	ID requested
⏵ 1	2020-11-01 22:47:41	announced

Dataset with its publication pending

submitter keyword: Human, Hematopoietic cancer cells, TMT, iDAPT

Jonathan Lee
contact affiliation	Cancer Research Institute, Beth Israel Deaconess Cancer Center Department of Medicine and Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
contact email	jdlee@post.harvard.edu
lab head
Jonathan Lee
contact affiliation	Harvard University
contact email	jdlee@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022252
     1. Label: PRIDE project
     2. Name: Dual DNA/protein tagging of open chromatin unveils dynamics of epigenomic landscapes in leukemia