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PXD022190-1

PXD022190 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrity of the short arm of nuclear pore Y-complex is required for mouse embryonic stem cell growth and differentiation
DescriptionNuclear pore complexes (NPCs) are established players in cell division and differentiation. However studies on the contribution of individual NPC subunits to these processes are still scarce. Here we have used mouse embryonic stem cells (mESCs) to characterize the role of structural components of the NPCs, focusing on the short arm of the Y-complex that comprises Nup85, Seh1 and Nup43. We show that Seh1 and Nup43, although dispensable at the pluripotent stage, are required for normal cell growth rates at that stage and for mESC viability upon differentiation. Lack of Seh1 or Nup43 in mESCs is associated with a mild reduction of NPC density that is also observed when Seh1 interaction with Nup85 is impaired. Nevertheless, mESC proliferation and differentiation are not altered in these ∆E2-GFP-Nup85 mutants, indicating that it is the integrity of the Y-complex, rather than the number of NPCs, that is critical to ensure these processes.
HostingRepositoryPRIDE
AnnounceDate2021-05-20
AnnouncementXMLSubmission_2021-05-20_00:53:15.103.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaurent Lignières
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-26 07:53:48ID requested
12021-05-20 00:53:15announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Nuclear pore, nucleoporin, Seh1, Nup43, Nup85, Mios, cell growth, cell differentiation, nuclear size, mouse ESCs
Contact List
Guillaume Chevreux
contact affiliationProteomic facility of Institut Jacques Monod (UMR7592, CNRS and Paris University, France)
contact emailGuillaume.CHEVREUX@ijm.fr
lab head
Laurent Lignières
contact affiliationProteomic facility of Institut Jacques Monod - CNRS & Université de Paris
contact emaillaurent.lignieres@ijm.fr
dataset submitter
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