PXD022190

PXD022190 is an original dataset announced via ProteomeXchange.

Title	Integrity of the short arm of nuclear pore Y-complex is required for mouse embryonic stem cell growth and differentiation
Description	Nuclear pore complexes (NPCs) are established players in cell division and differentiation. However studies on the contribution of individual NPC subunits to these processes are still scarce. Here we have used mouse embryonic stem cells (mESCs) to characterize the role of structural components of the NPCs, focusing on the short arm of the Y-complex that comprises Nup85, Seh1 and Nup43. We show that Seh1 and Nup43, although dispensable at the pluripotent stage, are required for normal cell growth rates at that stage and for mESC viability upon differentiation. Lack of Seh1 or Nup43 in mESCs is associated with a mild reduction of NPC density that is also observed when Seh1 interaction with Nup85 is impaired. Nevertheless, mESC proliferation and differentiation are not altered in these ∆E2-GFP-Nup85 mutants, indicating that it is the integrity of the Y-complex, rather than the number of NPCs, that is critical to ensure these processes.
HostingRepository	PRIDE
AnnounceDate	2021-05-20
AnnouncementXML	Submission_2021-05-20_00:53:15.103.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laurent Lignières
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 07:53:48	ID requested
⏵ 1	2021-05-20 00:53:15	announced

Dataset with its publication pending

submitter keyword: Nuclear pore, nucleoporin, Seh1, Nup43, Nup85, Mios, cell growth, cell differentiation, nuclear size, mouse ESCs

Guillaume Chevreux
contact affiliation	Proteomic facility of Institut Jacques Monod (UMR7592, CNRS and Paris University, France)
contact email	Guillaume.CHEVREUX@ijm.fr
lab head
Laurent Lignières
contact affiliation	Proteomic facility of Institut Jacques Monod - CNRS & Université de Paris
contact email	laurent.lignieres@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD022190

PRIDE project URI

• PRIDE
  1. PXD022190
     1. Label: PRIDE project
     2. Name: Integrity of the short arm of nuclear pore Y-complex is required for mouse embryonic stem cell growth and differentiation