PXD022138-1
PXD022138 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Native Elongin A Co-IP on mouse embryonic stem cells nuclear extract using R19 goat EloA antibody |
Description | To identify proteins associated with mouse Elongin A (EloA), we carried out Co-IP on nuclear extract prepared from mouse ES cells from wild-type and null Elongin A cells (negative control).Each immunoprecipitation was excised as 5 gel sections, minced and digested in-gel using trypsin. Electroionization was performed on the peptides before entering an LTQ Orbitrap Velos Pro ion trap mass spectrometer instrument (Thermo Fisher Scientific). Sequest (Thermo Finnigan, San Jose, CA) was used for identification of the peptide sequences. Identified peptides were filtered by false discovery rate of 1% or less. Processing, preparation, and Mass spectrometry analysis of the samples was carried out at Taplin Mass Spectrometry Facility (Harvard Medical School). |
HostingRepository | jPOST |
AnnounceDate | 2020-12-10 |
AnnouncementXML | Submission_2020-12-10_08:52:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Behfar Ardehali |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-10-22 20:06:25 | ID requested | |
⏵ 1 | 2020-12-10 08:52:26 | announced | |
2 | 2021-02-04 17:04:54 | announced | 2021-02-04: Updated FTP location. |
3 | 2022-09-18 03:18:11 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mouse ESC, Elongin A, Co-IP, LTQ Orbitrap Velos Pro ion trap mass spectrometer, |
Contact List
Robert Kingston | |
---|---|
lab head | |
Behfar Ardehali | |
contact affiliation | MGH |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://jpost.pharm.kyoto-u.ac.jp/repository/JPST000951 |