PXD022138

PXD022138 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Native Elongin A Co-IP on mouse embryonic stem cells nuclear extract using R19 goat EloA antibody
Description	To identify proteins associated with mouse Elongin A (EloA), we carried out Co-IP on nuclear extract prepared from mouse ES cells from wild-type and null Elongin A cells (negative control).Each immunoprecipitation was excised as 5 gel sections, minced and digested in-gel using trypsin. Electroionization was performed on the peptides before entering an LTQ Orbitrap Velos Pro ion trap mass spectrometer instrument (Thermo Fisher Scientific). Sequest (Thermo Finnigan, San Jose, CA) was used for identification of the peptide sequences. Identified peptides were filtered by false discovery rate of 1% or less. Processing, preparation, and Mass spectrometry analysis of the samples was carried out at Taplin Mass Spectrometry Facility (Harvard Medical School).
HostingRepository	jPOST
AnnounceDate	2020-12-10
AnnouncementXML	Submission_2022-09-18_03:18:11.188.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Behfar Ardehali
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-22 20:06:25	ID requested
1	2020-12-10 08:52:26	announced
2	2021-02-04 17:04:54	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 03:18:11	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: mouse ESC, Elongin A, Co-IP, LTQ Orbitrap Velos Pro ion trap mass spectrometer,

submitter keyword: mouse ESC, Elongin A, Co-IP, LTQ Orbitrap Velos Pro ion trap mass spectrometer,

Contact List

Robert Kingston
lab head
Behfar Ardehali
contact affiliation	MGH
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000951/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000951/