PXD022123-1
PXD022123 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Discovery of Klf2 Interactors in Mouse Embryonic Stem Cells by Immunoprecipitation-Mass Spectrometry Utilizing Exogenously Expressed Bait |
Description | Krüppel-like factor 2 (Klf2) is a DNA-binding transcription factor that regulates embryonic stem cell-specific gene expression. Transcription cofactors such as p300 acetyltransferases and Erk kinases interact with Klf2, providing an additional layer of transcription regulation in embryonic stem cells. To carry out a thorough survey of the Klf2 interactome in embryonic stem cells and identify novel transcription cofactors, we designed a modified immunoprecipitation-mass spectrometry (IP-MS) method. In this method, recombinant Klf2, expressed and purified from Sf9 insect cells instead of ectopically expressed in cells, was used as bait. Using this modified IP-MS method, we discovered nine Klf2-interacting proteins, including the previously reported Crebbp and p300. These proteins showed at least an 8-fold increase in signal intensity in Klf2 pull-downs compared with controls, with P-values < 0.010. Among the identified Klf2-binding proteins confirmed using our IP-MS workflow was Snd1, which we found to interact directly with Klf2 and function as a transcriptional coactivator of Klf2 to drive Oct4 gene expression. Collectively, our IP-MS protocol may offer a useful tool for identifying novel transcription cofactors in stem cells. |
HostingRepository | jPOST |
AnnounceDate | 2021-09-20 |
AnnouncementXML | Submission_2021-09-19_08:00:03.840.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yong-In Kim |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; alpha-aminocarbamoylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-10-21 21:34:18 | ID requested | |
⏵ 1 | 2021-09-19 08:00:05 | announced | |
2 | 2022-09-18 03:43:31 | announced | 2022-09-18: Updated FTP location. |
Publication List
Seo L, Kim YI, Kim H, Hyun K, Kim J, Lee JE, Discovery of Klf2 interactors in mouse embryonic stem cells by immunoprecipitation-mass spectrometry utilizing exogenously expressed bait. Biochim Biophys Acta Proteins Proteom, 1869(9):140672(2021) [pubmed] |
Keyword List
submitter keyword: Mouse embryonic stem cells |
Klf2 |
immunoprecipitation-mass spectrometry |
Snd1 |
exogenous bait |
Contact List
J. Eugene Lee | |
---|---|
lab head | |
Yong-In Kim | |
contact affiliation | Korea Research Institute of Standards and Science (KRISS) |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000987 |