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PXD022015-3

PXD022015 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of gastric tumor organoids with specific oncogenic pathway activations
DescriptionGastric cancer ranks the fifth most common malignancy and the third leading cause of cancer related death worldwide. Classical chemotherapy is still the standard treatment in advanced stages, with only two targeted therapies currently in clinical use. Several targeted trials have failed, likely due to missing relevant biomarkers that predict response. Patient derived cancer organoids (PDOs) constitute a three dimensional cell culture system showing self renewal, self organization and regularly unlimited proliferation while faithfully recapitulating many aspects of the tumor they are derived from. Nevertheless, the complex individual mutational landscape hampers the use of PDOs for basic and translational research. We therefore characterized three murine tumor organoid models with defined mutational patterns altering specific oncogenic pathways: a RAS activated (KrasG12D, Tp53R172H), a WNT activated (Apcfl/fl, Tp53R172H) and a diffuse (Cdh1fl/fl, Apcfl/fl) tumor model. The models were analyzed phenotypically, a proteome signature was established and a drug screen performed. The models were morphologically diverse, were characterized by individual protein expression signatures and showed differential drug sensitivities. The developed organoid models allow functional assays as well as pathway specific drug interference testing in a genetically defined setting.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:27.181.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFernando Garcia
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentQ Exactive; maXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-16 00:47:35ID requested
12022-08-22 04:51:12announced
22023-05-14 15:02:26announced2023-05-14: Updated project metadata.
32023-11-14 08:51:27announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Human proteome project, EPIC-XS
submitter keyword: phospho proteome signature, proteome signature, murine tumor organoid models,Gastric cancer
Contact List
Javier Muñoz Peralta
contact affiliationHead of Proteomics Unit Spanish National Cancer Research Centre (CNIO) Address: Melchor Fernández Almagro, 3. 28029 Madrid. SPAIN Phone: +34 917 328 000 Ext. 3110
contact emailjmunozpe@cnio.es
lab head
Fernando Garcia
contact affiliationFernando García Martínez, Ph.D. Proteomics Unit, Spanish National cancer Research Center, CNIO, MADRID, SPAIN
contact emailfgarcia@cnio.es
dataset submitter
Full Dataset Link List
Dataset FTP location
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