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PXD022013-1

PXD022013 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEvidence for structural protein damage and membrane lipid remodeling in red blood cells from COVID-19 patients
DescriptionThe SARS-CoV-2 beta coronavirus is the etiological driver of COVID-19 disease, which is primarily characterized by shortness of breath, persistent dry cough, and fever. Because they transport oxygen, red blood cells (RBCs) may play a role in the severity of hypoxemia in COVID-19 patients. The present study combines state-of-the-art metabolomics, proteomics, and lipidomics approaches to investigate the impact of COVID-19 on RBCs from 23 healthy subjects and 29 molecularly-diagnosed COVID-19 patients. RBCs from COVID-19 patients had increased levels of glycolytic intermediates, accompanied by oxidation and fragmentation of ankyrin, spectrin beta, and the N-terminal cytosolic domain of band 3 (AE1). Significantly altered lipid metabolism was also observed, especially short and medium chain saturated fatty acids, acyl-carnitines, and sphingolipids. Nonetheless, there were no alterations of clinical hematological parameters, such as RBC count, hematocrit, and mean corpuscular hemoglobin concentration, with only minor increases in mean corpuscular volume. Taken together, these results suggest a significant impact of SARS-CoV-2 infection on RBC structural membrane homeostasis at the protein and lipid levels. Increases in RBC glycolytic metabolites are consistent with a theoretically improved capacity of hemoglobin to offload oxygen as a function of allosteric modulation by high-energy phosphate compounds, perhaps to counteract COVID-19-induced hypoxia. Conversely, because the N-terminus of AE1 stabilizes deoxyhemoglobin and finely tunes oxygen off-loading and metabolic rewiring towards the hexose monophosphate shunt, RBCs from COVID-19 patients may be less capable to respond to environmental variations in hemoglobin oxygen saturation/oxidant stress when traveling from the lungs to peripheral capillaries and vice versa.
HostingRepositoryMassIVE
AnnounceDate2020-10-16
AnnouncementXMLSubmission_2020-10-16_07:48:13.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRyan Hill
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl; Gln->pyro-Glu; Acetyl
Instrumentinstrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-15 15:56:33ID requested
12020-10-16 07:48:13announced
Publication List
no publication
Keyword List
submitter keyword: SARS-CoV-2, erythrocyte, band 3, AE1, metabolomics, proteomics, lipidomics
Contact List
Angelo D'alessandro
contact affiliationUniversity of Colorado - Anschutz Medical Campus
contact emailAngelo.Dalessandro@cuanschutz.edu
lab head
Ryan Hill
contact affiliationUC-Denver
contact emailRyan.Hill@cuanschutz.edu
dataset submitter
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