PXD021985 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mapping sites of carboxymethyllysine modification on proteins reveals consequences for proteostasis and cell proliferation |
Description | Posttranslational mechanisms play a key role in modifying the abundance and function of cellular proteins. Among these, modification by advanced glycation end products (AGEs) has been shown to accumulate during aging and age-associated diseases but specific protein targets and functional consequences remain largely unexplored. Here, we devised a proteomic strategy to identify specific sites of carboxymethyllysine (CML) modification, one of the most abundant AGEs. We identified over 1000 sites of CML modification in mouse and primary human cells treated with the glycating agent glyoxal. By using quantitative proteomics, we found that protein glycation triggers a proteotoxic response and directly affects the protein degradation machinery. We show that glyoxal induces cell cycle perturbation of primary endothelial cells and that CML modification interferes with acetylation of tubulins and microtubule dynamics. Our data demonstrate the relevance of AGE modification for cellular function and pinpoints specific protein networks that might become compromised during aging. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-25 |
AnnouncementXML | Submission_2021-11-25_07:28:54.731.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alessandro Ori |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetic acid derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-14 23:32:03 | ID requested | |
⏵ 1 | 2021-11-25 07:28:55 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: advanced glycation end products, carboxymethyllysine, posttranslational modification, aging, proteasome, endothelial cell, cell cycle, tubulin, acetylation, mass spectrometry, liver, heart, kidney, glyoxal, mice, microtubules, mitosis |
Contact List
Alessandro Ori |
contact affiliation | Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), 07745 Jena, Germany |
contact email | alessandro.ori@leibniz-fli.de |
lab head | |
Alessandro Ori |
contact affiliation | Leibniz Institute on Aging |
contact email | aleori@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021985
- Label: PRIDE project
- Name: Mapping sites of carboxymethyllysine modification on proteins reveals consequences for proteostasis and cell proliferation