PXD021860-1
PXD021860 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic characterization of human neural stem cells during in vitro differentiation |
Description | One of the promising approaches to slow down or treat neurodegenerative diseases or spinal cord injuries represent cell therapies. The grafted cells could either integrate into the damaged tissue to replace dead or damaged cells or by secretion of different factors modulate inflammatory reaction, reduce tissue damage and support neuronal survival. However, due to the heterogeneity of in vitro cultured cells, comprehensive characterization of such cells is absolutely crucial to prevent safety risks. Here, we performed SWATH-MS analysis to characterize changes in proteome of human neural stem cells (NSCs) during their differentiation either spontaneously by withdrawal of EGF and FGF2 in cell culture media or by trophic support of BDNF/GDNF growth factors. We quantified about 2800 proteins over the 28 days of differentiation and showed that changes in cellular proteomes are caused mostly by differentiation time course, rather than type of differentiation itself and that the major changes in protein expression occurred between day 0 and day 7 of both differentiations. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2021-01-07 |
AnnouncementXML | Submission_2021-01-07_15:23:41.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jirina Tyleckova |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | Acetyl; Carboxymethyl; Carbamidomethyl; Oxidation |
Instrument | TripleTOF 5600+ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-10-06 11:03:38 | ID requested | |
⏵ 1 | 2021-01-07 15:23:42 | announced |
Publication List
Červenka J, Tylečková J, Kupcová Skalníková H, Vodičková Kepková K, Poliakh I, Valekova I, Pfeiferová L, Kolář M, Vaškovičová M, Pánková T, Vodička P. Proteomic characterization of human neural stem cells and their secretome during in vitro differentiation. Frontiers in Cellular Neuroscience. 2020;14:465. |
Keyword List
submitter keyword: NSCs, neural differentiation, SWATH-MS |
Contact List
Petr Vodička | |
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contact affiliation | Laboratory of Applied Proteome Analysis, Research Center PIGMOD, Institute of the Animal Physiology and Genetics of The Czech Academy of Sciences, Liběchov, Czech Republic |
contact email | vodicka@iapg.cas.cz |
lab head | |
Jirina Tyleckova | |
contact affiliation | Laboratory of Applied Proteome Analysis, Research Center PIGMOD, Institute of the Animal Physiology and Genetics of The Czech Academy of Sciences, Liběchov, Czech Republic |
contact email | tyleckova@iapg.cas.cz |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |