PXD021860

PXD021860 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of human neural stem cells during in vitro differentiation
Description	One of the promising approaches to slow down or treat neurodegenerative diseases or spinal cord injuries represent cell therapies. The grafted cells could either integrate into the damaged tissue to replace dead or damaged cells or by secretion of different factors modulate inflammatory reaction, reduce tissue damage and support neuronal survival. However, due to the heterogeneity of in vitro cultured cells, comprehensive characterization of such cells is absolutely crucial to prevent safety risks. Here, we performed SWATH-MS analysis to characterize changes in proteome of human neural stem cells (NSCs) during their differentiation either spontaneously by withdrawal of EGF and FGF2 in cell culture media or by trophic support of BDNF/GDNF growth factors. We quantified about 2800 proteins over the 28 days of differentiation and showed that changes in cellular proteomes are caused mostly by differentiation time course, rather than type of differentiation itself and that the major changes in protein expression occurred between day 0 and day 7 of both differentiations.
HostingRepository	PanoramaPublic
AnnounceDate	2021-01-07
AnnouncementXML	Submission_2021-01-07_15:23:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jirina Tyleckova
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Acetyl; Carboxymethyl; Carbamidomethyl; Oxidation
Instrument	TripleTOF 5600+

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-06 11:03:38	ID requested
⏵ 1	2021-01-07 15:23:42	announced

Červenka J, Tylečková J, Kupcová Skalníková H, Vodičková Kepková K, Poliakh I, Valekova I, Pfeiferová L, Kolář M, Vaškovičová M, Pánková T, Vodička P. Proteomic characterization of human neural stem cells and their secretome during in vitro differentiation. Frontiers in Cellular Neuroscience. 2020;14:465.

submitter keyword: NSCs, neural differentiation, SWATH-MS

Petr Vodička
contact affiliation	Laboratory of Applied Proteome Analysis, Research Center PIGMOD, Institute of the Animal Physiology and Genetics of The Czech Academy of Sciences, Liběchov, Czech Republic
contact email	vodicka@iapg.cas.cz
lab head
Jirina Tyleckova
contact affiliation	Laboratory of Applied Proteome Analysis, Research Center PIGMOD, Institute of the Animal Physiology and Genetics of The Czech Academy of Sciences, Liběchov, Czech Republic
contact email	tyleckova@iapg.cas.cz
dataset submitter

Panorama Public dataset URI