PXD021789-1
PXD021789 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The AMBRA1 E3 ligase adaptor regulates Cyclin D protein stability |
Description | Here we describe AMBRA1 as the main regulator of Cyclin D protein degradation. We first identify AMBRA1 as the top candidate in a genome-wide CRISPR/Cas9 loss-of-function screen investigating the genetic basis of resistance to chemical CDK4/6 inhibition. AMBRA1 loss results in high protein levels of Cyclin D in cells and in mice. AMBRA1 loss further promotes lung cancer development in a mouse model, and low levels of AMBRA1 correlate with worse survival in lung cancer patients. Mechanistically, AMBRA1 acts as a substrate receptor for the Cullin 4 E3 ligase complex to promote ubiquitylation and proteasomal degradation of the three Cyclin D family members. Thus, AMBRA1 regulates Cyclin D protein levels and contributes to the development of cancer as well as the response of cancer cells to CDK4/6 inhibitors. |
HostingRepository | PRIDE |
AnnounceDate | 2021-07-24 |
AnnouncementXML | Submission_2021-07-24_11:45:49.636.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021789 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Janos Demeter |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-10-02 04:14:56 | ID requested | |
⏵ 1 | 2021-07-24 11:45:50 | announced |
Publication List
Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R, Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J, D. Nature, 592(7856):794-798(2021) [pubmed] |
Keyword List
submitter keyword: Human, Cell cycle, Cyclin D, cancer |
Contact List
Julien Sage | |
---|---|
contact affiliation | Departments of Pediatrics and Genetics, Stanford University, Stanford, CA, USA. |
contact email | julsage@stanford.edu |
lab head | |
Janos Demeter | |
contact affiliation | Stanford University |
contact email | jdemeter@stanford.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD021789 |
PRIDE project URI |
Repository Record List
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