PXD021711-1

PXD021711 is an original dataset announced via ProteomeXchange.

Title	Proteomics reveals virulence-related proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii yeast cells grown in the presence of L-dihydroxyphenylalanine
Description	Species of the genus Paracoccidioides cause a systemic infection in human patients. Yeast cells of Paracoccidioides spp. produce melanin in the presence of L-dihydroxyphenylalanine and during infection, which may impact the pathogen survival into the host. To better understand the metabolic changes that occur in melanized Paracoccidioides spp. cells, a proteomic approach was performed to compare melanized and non-melanized Paracoccidioides brasiliensis and Paracoccidioides lutzii yeast cells. Melanization was conducted using L-dihydroxyphenylalanine as a precursor and quantitative proteomics was performed using reversed-phase chromatography coupled to high resolution mass spectrometry. When comparing melanized versus non-melanized cells, 999 and 577 differentially abundant proteins were identified for P. brasiliensis and P. lutzii, respectively. Functional enrichment and comparative analysis revealed 30 abundant biological processes in melanized P. brasiliensis and 18 in P. lutzii, while non-melanized cells from these species had 21 and 25 differentially abundant processes, respectively. Melanized cells presented abundance of other virulence-associated proteins, such as phospholipase, proteases, superoxide dismutase, heat-shock proteins, as well as proteins related to cell-wall remodeling and vesicular transport. The results suggest that L-dihydroxyphenilalanine increases virulence of Paracoccidioides spp. through a complex mechanism involving not only melanin, but other virulence factors as well.
HostingRepository	PRIDE
AnnounceDate	2021-01-20
AnnouncementXML	Submission_2021-01-19_22:08:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Hemmi Valente
SpeciesList	scientific name: Paracoccidioides brasiliensis Pb18; NCBI TaxID: 502780; scientific name: Paracoccidioides lutzii Pb01; NCBI TaxID: 502779;
ModificationList	iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-28 06:01:42	ID requested
⏵ 1	2021-01-19 22:08:10	announced

Almeida-Paes R, Almeida MA, Baeza LC, Marmello LAM, Trugilho MRO, Nosanchuk JD, Soares CMA, Valente RH, Zancop, é, -Oliveira RM, Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine. J Fungi (Basel), 6(4):(2020) [pubmed]

submitter keyword: Paracoccidioides, melanin, L-dihydroxyphenylalanine, proteomics, virulence

Rodrigo Almeida-Paes
contact affiliation	Mycology Laboratory, Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Brazil
contact email	rodrigo.paes@ini.fiocruz.br
lab head
Richard Hemmi Valente
contact affiliation	Fundação Oswaldo Cruz
contact email	richardhemmi@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021711
     1. Label: PRIDE project
     2. Name: Proteomics reveals virulence-related proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii yeast cells grown in the presence of L-dihydroxyphenylalanine