PXD021661-2

PXD021661 is an original dataset announced via ProteomeXchange.

Title	TMTPro Complementary Ion Quantification Increase Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 level
Description	The ability of the TMTPro isobaric labeling reagents to form complementary ions for accurate multiplexed proteomics at the MS2 level was investigated. Human and yeast peptides were labeled in distinct ratios to analyze the effect of interference on the quantification accuracy. A method, TMTProC, was developed and optimized for accurate, sensitive MS2-level quantification of up to 8 conditions in one MS-run.
HostingRepository	PRIDE
AnnounceDate	2021-05-11
AnnouncementXML	Submission_2021-05-10_22:45:15.100.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021661
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Stadlmeier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	N-ethylmaleimide derivatized cysteine; monohydroxylated residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-24 01:23:32	ID requested
1	2021-05-10 21:21:26	announced
⏵ 2	2021-05-10 22:45:15	announced	2021-05-11: Updated project metadata.

Johnson A, Stadlmeier M, W, ü, hr M, TMTpro Complementary Ion Quantification Increases Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 Level. J Proteome Res, 20(6):3043-3052(2021) [pubmed]

10.1021/ACS.JPROTEOME.0C00813;

submitter keyword: complementary ion quantification, interference-free quantification, multiplexing,TMTpro

Martin Wühr
contact affiliation	Assistant Professor of Molecular Biology and the Lewis-Sigler Institute for Integrative Genomics, Princeton University
contact email	wuhr@princeton.edu
lab head
Michael Stadlmeier
contact affiliation	Princeton University
contact email	ms93@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD021661

PRIDE project URI

• PRIDE
  1. PXD021661
     1. Label: PRIDE project
     2. Name: TMTPro Complementary Ion Quantification Increase Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 level