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PXD021661 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTMTPro Complementary Ion Quantification Increase Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 level
DescriptionThe ability of the TMTPro isobaric labeling reagents to form complementary ions for accurate multiplexed proteomics at the MS2 level was investigated. Human and yeast peptides were labeled in distinct ratios to analyze the effect of interference on the quantification accuracy. A method, TMTProC, was developed and optimized for accurate, sensitive MS2-level quantification of up to 8 conditions in one MS-run.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichael Stadlmeier
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListN-ethylmaleimide derivatized cysteine; monohydroxylated residue
InstrumentOrbitrap Eclipse; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-24 01:23:32ID requested
12021-05-10 21:21:26announced
22021-05-10 22:45:15announced2021-05-11: Updated project metadata.
Publication List
Johnson A, Stadlmeier M, Wühr M, TMTpro Complementary Ion Quantification Increases Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 Level. J Proteome Res, 20(6):3043-3052(2021) [pubmed]
Keyword List
submitter keyword: complementary ion quantification, interference-free quantification, multiplexing,TMTpro
Contact List
Martin Wühr
contact affiliationAssistant Professor of Molecular Biology and the Lewis-Sigler Institute for Integrative Genomics, Princeton University
contact emailwuhr@princeton.edu
lab head
Michael Stadlmeier
contact affiliationPrinceton University
contact emailms93@princeton.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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