PXD021646

PXD021646 is an original dataset announced via ProteomeXchange.

Title	The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes
Description	STRIPAK is a highly conserved eukaryotic signalling complex controlling a wide range of developmental processes in microorganisms as well as humans. This study uses iTRAQ-based proteomic and phosphoproteomic analyses to expand the identification of differentially phosphorylated proteins, comparing the wildtype proteome of the model fungus S. macorspora to those from three STRIPAK single- and double deletion mutants. Among the differentially phosphorylated proteins, we detected Gul1, a homolog of GUL-1 from N. crassa and SSD1 from yeast. In this study, we further provide a functional analysis of Gul1, showing it controls sexual development and hyphal morphology in a phosphorylation dependent manner.
HostingRepository	PRIDE
AnnounceDate	2020-09-24
AnnouncementXML	Submission_2020-09-24_01:50:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bernhard Blank-Landeshammer
SpeciesList	scientific name: Sordaria macrospora k-hell; NCBI TaxID: 771870;
ModificationList	phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-23 06:56:51	ID requested
⏵ 1	2020-09-24 01:50:32	announced

Dataset with its publication pending

submitter keyword: Sordaria macrospora, LC-MSMS, Phosphoproteomics, iTRAQ

Prof. Dr. Albert Sickmann
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact email	sickmann@isas.de
lab head
Bernhard Blank-Landeshammer
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS e.V.
contact email	blank.bernhard@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD021646

PRIDE project URI

• PRIDE
  1. PXD021646
     1. Label: PRIDE project
     2. Name: The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes