PXD021622-1

PXD021622 is an original dataset announced via ProteomeXchange.

Title	Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling
Description	Galectin-3 is a glycan-binding protein (GBP) that binds β -galactoside glycan structures to orchestrate a variety of important biological events, including the activation of hepatic stellate cells and regulating immunological responses. While the requisite glycan epitopes needed to bind galectin-3 have long been elucidated, the cellular glycoproteins that bear these glycan signatures remain unknown. Given the importance of the three-dimensional arrangement of glycans in dictating GBP interactions, strategies that allow the identification of GBP receptors in live cells, where the native glycan presentation and glycoprotein expression are preserved, possess significant advantages over static and artificial systems. Here, we describe the integration of a proximity labeling method and quantitative mass spectrometry to map the glycan and glycoprotein interactors for galectin-3 in live human hepatic stellate cells and peripheral blood mononuclear cells. Understanding the identity of the glycoproteins and defining the structures of the glycans will empower efforts to design and develop selective therapeutics to mitigate galectin-3-mediated biological events.
HostingRepository	PRIDE
AnnounceDate	2021-06-16
AnnouncementXML	Submission_2021-06-15_22:33:01.865.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eugene Joeh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-22 06:47:24	ID requested
⏵ 1	2021-06-15 22:33:02	announced

Joeh E, O'Leary T, Li W, Hawkins R, Hung JR, Parker CG, Huang ML, Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling. Proc Natl Acad Sci U S A, 117(44):27329-27338(2020) [pubmed]

submitter keyword: galectins, proteomics, proximity labeling

Mia L. Huang
contact affiliation	Department of Molecular Medicine Department of Chemistry The Scripps Research Institute
contact email	miahuang@scripps.edu
lab head
Eugene Joeh
contact affiliation	The Scripps Research Institute
contact email	ejoeh@scripps.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021622
     1. Label: PRIDE project
     2. Name: Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling